National Repository of Grey Literature 36 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Screening for systemic amyloidoses in endoscopic biopsy samples.
Květoň, Martin ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in endoscopic biopsy samples BACKGROUND Systemic amyloidoses constitute a complex and heterogeneous group of serious diseases that result from protein misfolding and whose common feature is the deposition of pathological protein substances (amyloid) in the extracellular space of tissues. Diagnostics of amyloidoses is based on histopathological examination of tissue samples and direct demonstration of the presence of amyloid deposits. The overall purpose of this diploma thesis was to create, apply and subsequently evaluate a screening program focused on systemic amyloidoses. METHODS In the first part of the experimental study, available staining techniques used for the detection of amyloid deposits in the tissue were compared. The second part of the experimental study consisted of performing screening examinations on endoscopic biopsy samples of the gastrointestinal tract. RESULTS Based on the results of comparison, a method employing the Sirius red F3B dye was chosen as a suitable screening technique....
Screening for systemic amyloidoses in endoscopic biopsy samples.
Květoň, Martin ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in endoscopic biopsy samples BACKGROUND Systemic amyloidoses constitute a complex and heterogeneous group of serious diseases that result from protein misfolding and whose common feature is the deposition of pathological protein substances (amyloid) in the extracellular space of tissues. Diagnostics of amyloidoses is based on histopathological examination of tissue samples and direct demonstration of the presence of amyloid deposits. The overall purpose of this diploma thesis was to create, apply and subsequently evaluate a screening program focused on systemic amyloidoses. METHODS In the first part of the experimental study, available staining techniques used for the detection of amyloid deposits in the tissue were compared. The second part of the experimental study consisted of performing screening examinations on endoscopic biopsy samples of the gastrointestinal tract. RESULTS Based on the results of comparison, a method employing the Sirius red F3B dye was chosen as a suitable screening technique....
Acute and chronic inflammatory diseases of the skeletal system
Havránková, Barbora ; Němečková, Ivana (advisor) ; Kudláčková, Zděnka (referee)
NAME: Acute and chronic inflammatory diseases of skeletal system AUTHOR: Bc. Barbora Havránková DEPARTEMENT: Department od Biological and Medical Sciences SUPERVISOR: RNDr. Ivana Němečková, Ph.D. This diploma thesis summaries informations about acute and chronic inflammatory diseases of the skeletal system. In the first part the skeletal system is described and agents of disease are analyzed. The most common agents are staphylococci, especially Staphylococcus aureus, streptococci, pneumococci and Haemophilus influenzae. In the next part the individual diseases are analyzed, the methods of their diagnosis and therapy are mentioned. Diagnosis of inflammatory diseases of the skeletal system is often complicated and is based on a detailed anamnesis. For all the diseases, the therapy consists of pharmacological, surgical and complementary therapy. All these diseases cause pain and often complicate life. Basic diagnostic methods used for diagnosis are imaging examination methods such as RTG, CT, MR or scintigraphy. Laboratory examinations are appropriate but not always authoritative. The treatment of inflammatory diseases of the skeleton is based on non-steroidal anti- rheumatic drugs, disease modifying antirheumatic drug (DMARDs), antibiotics and biological drugs. KEY WORDS: skeletal system,...
Diagnostics of Hodgkin's lymphoma
Gabriel, Kamila ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
AUTHOR Kamila Gabriel TITLE Diagnosis of Hodgkin's lymphoma DEPARTMENT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences SUPERVISOR prof. PharmDr. Petr Nachtigal, Ph.D. ABSTRACT In 2015, almost three thousand of new cases of leukemias and lymphomas were reported and 1751 patients died from these cancers in the Czech Republic. Out of these, 277 cases (9 %) and 60 deaths (3.5 %) were related to Hodgkin's lymphoma (HL). In Hodgkin's lymphoma the tumor cells comprise only a small minority of the involved tissue. Two distinct groups of HL are recognized: Classical Hodgkin's Lymphoma (CHL), further divided into four subtypes, and Nodular Lymphocyte-Predominant Hodgkin's Lymphoma (NLPHL). CHL affects primarily cervical lymph nodes of young adults and may spread to other sites. The prognosis is very good with 80-90 % of patients being cured by radiotherapy and multiagent chemotherapy. However, these patients are at a significant risk of secondary malignancies. NLPHL, which constitutes about 5 % of HL, affects primarily cervical and axillary lymph nodes of patients between 30 and 40 years of age. Usually, the progression is slow and the tumor shows excellent sensitivity to therapy. When a suspicion of lymphoproliferative disease arises it is recommended...
Morphological characteristics of alterations in lungs in a mouse model of atherosclerosis.
Vyskočilová, Martina ; Němečková, Ivana (advisor) ; Blažíčková, Kateřina (referee)
Bachelor thesis Morphological characteristics of alterations in lungs in a mouse model of atherosclerosis Author: Martina Vyskočilová Study course: Medical laboratory technician Charles University Faculty of Pharmacy in Hradec Králové Background: Atherosclerosis is a multifactorial disease caused by deposition of blood lipids into the intima layer of blood vessels. Due to the fact that process of atherogenesis is associated with immune reactions, disease is considered as an inflammatory process. Atherosclerotic plaques rarely affect pulmonary arteries, where this condition is accompanied by an increase of pulmonary pressure, which is very likely the trigger of disease. Aim of this study is to find out whether there are morphological changes in the lungs of ApoE/LDLr-/- mouse model of atherosclerosis, characteristic for pulmonary atherosclerosis, compared to lungs of healthy mice. Methods: Lungs of ApoE/LDLr-/- and healthy C57BL/6J control mice have been fixed in 4% neutral formaldehyde, embedded in paraffin and sliced into 4 μm thin sections. One of the sections has been stained with hematoxylin-eosin, the other one with green trichrome. Staining with hematoxylin-eosin is primary staining used for basic imaging of histological structure of tissues, staining with green trichrome is used for imaging...
Impact of spermiogram quality on in vitro fertilisation
Gold, Lukáš ; Němečková, Ivana (advisor) ; Kudláčková, Zděnka (referee)
Impact of spermiogramme quality on in vitro fertilization Master's Thesis Author: Bc. Lukáš Gold Field of study: Specialist in laboratory methods Charle's university in Prague Faculty of Pharmacy in Hradec Králové Number of couples who have problems with becoming pregnant increasis every year. Sometimes, change of lifestyle or alleviation of life tempo can help but in other cases couples have to use centre of assisted reproduction service. In some cases is inferility caused by only one factor, in other cases are factors combinated. The aim of this work was evaluation of question, if the spermiogramme quality influences quality of artificial insemination. The secondary aim was to evaluate which technique of oocyte active insemination by injection (Intracytoplasmic sperm injection, ICSI or Intracytoplasmic morphologically - selected sperm injection IMSI) is more successful at fertilization in vitro. Datas and results were taken from 100 couples who underwent therapy using donated oocytes at Zentren Prof. Zech - Pilsen, s.r.o. clinic. Men who gave a fresh ejaculate in day of oocyte collection by ovarian puncture are included in this work. Donated oocytes were micromanipulatively impregnated by spemrs which were taken from ejaculate. Acording to the spermiogramme results men were divided into two...
Progression of endothelial dysfunction in heart in a mouse model of atherosclerosis.
Filipová, Petra ; Němečková, Ivana (advisor) ; Doleželová, Eva (referee)
Charles University in Prague, Faculty od Pharmacy in Hradec Králové Department of Biological and Medical Sciences Progression of endothelial dysfunction in heart in a mouse model of atherosclerosis Author of diploma thesis: Petra Filipová Supervisor: RNDr. Ivana Němečková, Ph.D. Backround: Atherosclerosis is a disease when atherosclerotic lesions are formed in vessels. It represents the major cause of the heart failure and stroke. The endothelial dysfunction, which has been recorded in a large number of animals, is considered one of the main causes of atherosclerosis. In our work we have focused on the detection of the endothelial dysfunction markers in the heart of the ApoE/LDLR double knock-out mouse model. Methods: We detected changes in the expression of eNOS, p-eNOS and HO-1 in two-months and eight-months old ApoE/LDLR double knock-out mice, which were fed standard chow diet. For the semiquantitative evaluation of the observed markers we used the Western blot analysis of the homogenated left ventricle. Results: According to our results there is no difference in the expression of eNOS, p-eNOS and HO-1 in the studied mouse model of atherosclerosis in relation to age. This might mean, that the endothelial dysfunction in the coronary vessels of these mice doesn't develop. However, for further...
Optimalisation of methods for determination of CD44 receptor activation
Skalická, Nela ; Němečková, Ivana (advisor) ; Konečná, Klára (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of diploma thesis: Optimalisation of methods for determination of CD44 receptor activation. CD44 is a transmembrane glycoprotein present in most cell types which is involved in many physiological and pathological processes and it represents the main hyaluronic acid (HA) receptor. HA is a nonsulfated glycosaminoglycan that is present extensively in extracellular matrix, where it provides cell hydratation. HA is involved in cancer, affects cell migration, supports an angiogenesis and participates in wound healing. In this study, we focused on the optimization of methods for the evaluation of activation, the amount and cleavage of CD44 receptor in HT-29 cell line. We observed the total amount of the receptor and its cleaved domains using methods Western Blot, ELISA and flow cytometry. We used HA molecular weight 71 kDa and PMA (Phorbol- 12-Myristate-13-Acetate) for activation. Time-dependent changes appeared in the amount of total CD44 and its fragments after activation. We also observed cell death depending on the presence of HA and PMA. We demonstrated no significant effects of these substances on cell viability. We used fluorescent and confocal microscopy for visual evaluation of...
Detection of inflammation in heart in a mouse model of atherosclerosis
Dlouhá, Michaela ; Němečková, Ivana (advisor) ; Kovařík, Miroslav (referee)
Detection of heart inflammation in a mouse model of atherosclerosis Diploma thesis Author: Michaela Dlouhá Study program: Pharmacy Charles University in Prague Faculty of Pharmacy in Hradec Králové Background: Atherosclerosis is an inflammatory disease caused by deposition of blood lipids in the wall of arteries involving a wide range of inflammatory substances. Recent evidences suggest, that inflammation plays an irreplaceable role in the development and subsequent complications of this disease. Raising proinflammatory markers increase worsening of prognosis in patients with acute coronary syndrome, indepently of myocardial damage. Therefore, the aim of this study is to compare the changes in the expression of inflammatory markers in ApoE/LDLr-/- mouse model of atherosclerosis. Methods: By the Western blot method we monitored the expression of VCAM-1, ICAM-1 and P-selectin in the myocardium of 2 and 8 months old ApoE/LDLr-/- mice fed by standard diet. Our results show, that we did not detect any statistically significant differences in expression of observed markers. Results and conclusions: Based on the results we assume, that there is a deterioration of the inflammatory response in myocardial ApoE/LDLr-/- mice. However, for a more detailed description of coronary dysfunction in this model, we...
Imunological and histological methods in Huntington's disease research
Jandurová, Patrícia ; Němečková, Ivana (advisor) ; Rathouská, Jana (referee)
Author: Patrícia Jandurová Title of thesis: Imunologické a histologické metody ve výzkumu Huntingtonovy nemoci Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study course: Medical laboratory technician Huntington's disease (HD) is a severe autosomal inherited neurodegenerative disorder that affects individuals regardless of gender. This disorder is caused by an expansion of CAG repeats in huntingtin gene, that is expressed in almost all tissues. Mutant protein causes a neuron degeneration in a brain of HD pacients which leads to a motor abnormalities and personality decay. The disease developes in the presence of a mutation in one of two huntingin alleles. The risk of transmission of disease for the direct descendants is 50%. This disease is very malign because of its late onset. An adequate therapy does not exist yet, but a lot of research teams focus on designig a suitable medical treatment. This work is devoted to the description of Huntington's disease and is focused on immunological and histological methods used in animal model experimental studies. Particular techniques are described and the results are supported with figures. Key words: Huntington's disease, CAG repeat, immunological and histological methods, animal model

National Repository of Grey Literature : 36 records found   1 - 10nextend  jump to record:
See also: similar author names
3 Němečková, Ilona
2 Němečková, Iva
1 Němečková, Iveta
Interested in being notified about new results for this query?
Subscribe to the RSS feed.