National Repository of Grey Literature 41 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Soluble endoglin effects on endothelial dysfunction markers in mouse aorta
Kalmanová, Lenka ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Soluble endoglin effects on endothelial dysfunction markers in mouse aorta Author of Diploma Thesis: Lenka Kalmanová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of the presented diploma thesis was to identify and subsequently evaluate the expression of endothelial dysfunction markers (ICAM-1, VCAM-1, P- selectin) in aortas of two groups of transgenic mice. The difference between control and experimental group was in the level of human soluble endoglin. Methods: 12 months old male mice of transgenic strain CBAxC57BL/6J with inserted genetic information for the expression of human soluble endoglin were utilized for the experiment. The mice were divided into two groups - the experimental group (high levels of sEng) and the control group (low levels of sEng) by ELISA analysis of levels of sEng in plasma. Both groups of mice were fed with standard laboratory rodent diet. Western blot analysis of adhesion molecules ICAM-1, VCAM-1 and P-selectin in mouse aorta and subsequently ELISA analysis of plasma levels of sVCAM-1 molecule were performed. Total cholesterol and triacylglycerol levels were demonstrated by biochemical analysis....
Study of the variations in the expression of different adhesion and cytoskeletal proteins of podocytes (E-Cadherin, Podocin, Vimentin) due to Bisphenol A
Chvojanová, Zuzana ; Kovařík, Miroslav (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové, Department of Biological and Medical Sciences The University of Alcalá, Faculty of Medicine, Department of biomedicine and biotechnology Student: Zuzana Chvojanová Supervisor: PharmDr. Miroslav Kovařík, Ph.D. Consultant: María Isabel Arenas Jimenéz Title of the diploma thesis: Study of the variations in the expression of different adhesion and cytoskeletal proteins of podocytes (E-Cadherin, Podocin, Vimentin) due to Bisphenol A Bisphenol A (BPA) is one of the most widespread compounds in the world, producing over 6 billion metric tons per year. It is widely used as part of polycarbonate plastics and epoxy resins, from which reusable plastic bottles, food boxes and some medical equipment are made. It is also used to coat the inner layer of the cans. Previous studies have shown that BPA contributes to many chronic diseases in the human body, such as kidney disease - diabetic nephropathy. Podocytes - terminally differentiated cells of the Bowman's capsule in glomerulus - are an integral part of the filtration barrier, where they play an important role in preventing the plasmatic proteins from penetrating to the urine. Therefore, in this study, we looked at the effect of BPA on these cells and their particular proteins, using both in vivo and...
Soluble endoglin effects on endothelial dysfunction markers in mouse aorta
Kalmanová, Lenka ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Soluble endoglin effects on endothelial dysfunction markers in mouse aorta Author of Diploma Thesis: Lenka Kalmanová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of the presented diploma thesis was to identify and subsequently evaluate the expression of endothelial dysfunction markers (ICAM-1, VCAM-1, P- selectin) in aortas of two groups of transgenic mice. The difference between control and experimental group was in the level of human soluble endoglin. Methods: 12 months old male mice of transgenic strain CBAxC57BL/6J with inserted genetic information for the expression of human soluble endoglin were utilized for the experiment. The mice were divided into two groups - the experimental group (high levels of sEng) and the control group (low levels of sEng) by ELISA analysis of levels of sEng in plasma. Both groups of mice were fed with standard laboratory rodent diet. Western blot analysis of adhesion molecules ICAM-1, VCAM-1 and P-selectin in mouse aorta and subsequently ELISA analysis of plasma levels of sVCAM-1 molecule were performed. Total cholesterol and triacylglycerol levels were demonstrated by biochemical analysis....
Morphological alterations in kidneys in mice with high soluble endoglin levels
Vyskočilová, Martina ; Němečková, Ivana (advisor) ; Nachtigal, Petr (referee)
Masters thesis Morphological alterations in kidneys in mice with high soluble endoglin levels Author: Bc. Martina Vyskočilová Study program: Medical bioanalytics Charles University Faculty of Pharmacy in Hradec Králové Background: Endoglin (Eng) is a transmembrane glycoprotein also known as an accesory receptor type III for a TGF-β molecule which is expressed primarily on the surface of endothelial cells. Endoglin plays a role in vascular homeostasis, angiogenesis and cardiovascular development. Soluble endoglin (sEng) which is formed by a cleavage of an extracelullar part of a membrane-bound endoglin scavenges the TGF-β molecules in the serum and blocks their binding to the membrane-bound receptor. Soluble endoglin is considered as a trigger of an endothelial dysfunction which is associated with cardiovascular diseases and patogenesis of preeclampsia. The aim of this study is to describe possible effect of high levels of a soluble endoglin on kidney tissue of transgenic mice in comparison with kidneys of mice with low levels of a human soluble endoglin. Methods: Kidneys of twelve months old mice with high and normal levels of a human soluble endoglin on a standard laboratory diet were fixed in 4% formaldehyde, embedded in paraffin and sliced into 7 μm thin sections. One of these sections was...
Daunorubicin effects on atherogenesis in rabbits fed by cholesterol diet.
Vašinová, Martina ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Daunorubicin (DAU) was the first anthracycline (ANT) antibiotic with impressive clinical activity in the treatment of acute pediatric leukemias. Later, it has been shown that anthracyclines have broad range of therapeutic activities against both hematological and solid tumors, including breast, ovarian and gastric carcinoma. On the other hand, ANTs are the most dangerous anticancer drugs with respect to cardiovascular toxicity characterized by mostly irreversible morphological changes and cell death. Indeed, ANTs are used also in patients with hypercholesterolemia so the effect on atherogenesis in blood vessels is of interest. Thus, we hypothesized that DAU treatment and cholesterol diet will aggravate atherosclerosis when compared to rabbits fed by cholesterol diet only. Twelve adult male New Zealand White rabbits were fed with cholesterol diet (0.2% cholesterol). Control group of rabbits (n=7) received saline i.v. once weekly for 10 weeks since the beginning of the 4th week, the second DAU group (n=7) received clinically relevant doses of daunorubicin (3 mg/kg, ≈ 50 mg/m2, i.v.). The systematic uniform random sampling was applied for the quantification of atherosclerotic lesions. The samples from aortic arch were taken for the evaluation. Serial sections of vessel (7 μm) were cut on a cryostat...
Screening for systemic amyloidoses in endoscopic biopsy samples.
Květoň, Martin ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in endoscopic biopsy samples BACKGROUND Systemic amyloidoses constitute a complex and heterogeneous group of serious diseases that result from protein misfolding and whose common feature is the deposition of pathological protein substances (amyloid) in the extracellular space of tissues. Diagnostics of amyloidoses is based on histopathological examination of tissue samples and direct demonstration of the presence of amyloid deposits. The overall purpose of this diploma thesis was to create, apply and subsequently evaluate a screening program focused on systemic amyloidoses. METHODS In the first part of the experimental study, available staining techniques used for the detection of amyloid deposits in the tissue were compared. The second part of the experimental study consisted of performing screening examinations on endoscopic biopsy samples of the gastrointestinal tract. RESULTS Based on the results of comparison, a method employing the Sirius red F3B dye was chosen as a suitable screening technique....
Screening for systemic amyloidoses in endoscopic biopsy samples.
Květoň, Martin ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in endoscopic biopsy samples BACKGROUND Systemic amyloidoses constitute a complex and heterogeneous group of serious diseases that result from protein misfolding and whose common feature is the deposition of pathological protein substances (amyloid) in the extracellular space of tissues. Diagnostics of amyloidoses is based on histopathological examination of tissue samples and direct demonstration of the presence of amyloid deposits. The overall purpose of this diploma thesis was to create, apply and subsequently evaluate a screening program focused on systemic amyloidoses. METHODS In the first part of the experimental study, available staining techniques used for the detection of amyloid deposits in the tissue were compared. The second part of the experimental study consisted of performing screening examinations on endoscopic biopsy samples of the gastrointestinal tract. RESULTS Based on the results of comparison, a method employing the Sirius red F3B dye was chosen as a suitable screening technique....
Acute and chronic inflammatory diseases of the skeletal system
Havránková, Barbora ; Němečková, Ivana (advisor) ; Kudláčková, Zděnka (referee)
NAME: Acute and chronic inflammatory diseases of skeletal system AUTHOR: Bc. Barbora Havránková DEPARTEMENT: Department od Biological and Medical Sciences SUPERVISOR: RNDr. Ivana Němečková, Ph.D. This diploma thesis summaries informations about acute and chronic inflammatory diseases of the skeletal system. In the first part the skeletal system is described and agents of disease are analyzed. The most common agents are staphylococci, especially Staphylococcus aureus, streptococci, pneumococci and Haemophilus influenzae. In the next part the individual diseases are analyzed, the methods of their diagnosis and therapy are mentioned. Diagnosis of inflammatory diseases of the skeletal system is often complicated and is based on a detailed anamnesis. For all the diseases, the therapy consists of pharmacological, surgical and complementary therapy. All these diseases cause pain and often complicate life. Basic diagnostic methods used for diagnosis are imaging examination methods such as RTG, CT, MR or scintigraphy. Laboratory examinations are appropriate but not always authoritative. The treatment of inflammatory diseases of the skeleton is based on non-steroidal anti- rheumatic drugs, disease modifying antirheumatic drug (DMARDs), antibiotics and biological drugs. KEY WORDS: skeletal system,...
Diagnostics of Hodgkin's lymphoma
Gabriel, Kamila ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
AUTHOR Kamila Gabriel TITLE Diagnosis of Hodgkin's lymphoma DEPARTMENT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences SUPERVISOR prof. PharmDr. Petr Nachtigal, Ph.D. ABSTRACT In 2015, almost three thousand of new cases of leukemias and lymphomas were reported and 1751 patients died from these cancers in the Czech Republic. Out of these, 277 cases (9 %) and 60 deaths (3.5 %) were related to Hodgkin's lymphoma (HL). In Hodgkin's lymphoma the tumor cells comprise only a small minority of the involved tissue. Two distinct groups of HL are recognized: Classical Hodgkin's Lymphoma (CHL), further divided into four subtypes, and Nodular Lymphocyte-Predominant Hodgkin's Lymphoma (NLPHL). CHL affects primarily cervical lymph nodes of young adults and may spread to other sites. The prognosis is very good with 80-90 % of patients being cured by radiotherapy and multiagent chemotherapy. However, these patients are at a significant risk of secondary malignancies. NLPHL, which constitutes about 5 % of HL, affects primarily cervical and axillary lymph nodes of patients between 30 and 40 years of age. Usually, the progression is slow and the tumor shows excellent sensitivity to therapy. When a suspicion of lymphoproliferative disease arises it is recommended...
Morphological characteristics of alterations in lungs in a mouse model of atherosclerosis.
Vyskočilová, Martina ; Němečková, Ivana (advisor) ; Blažíčková, Kateřina (referee)
Bachelor thesis Morphological characteristics of alterations in lungs in a mouse model of atherosclerosis Author: Martina Vyskočilová Study course: Medical laboratory technician Charles University Faculty of Pharmacy in Hradec Králové Background: Atherosclerosis is a multifactorial disease caused by deposition of blood lipids into the intima layer of blood vessels. Due to the fact that process of atherogenesis is associated with immune reactions, disease is considered as an inflammatory process. Atherosclerotic plaques rarely affect pulmonary arteries, where this condition is accompanied by an increase of pulmonary pressure, which is very likely the trigger of disease. Aim of this study is to find out whether there are morphological changes in the lungs of ApoE/LDLr-/- mouse model of atherosclerosis, characteristic for pulmonary atherosclerosis, compared to lungs of healthy mice. Methods: Lungs of ApoE/LDLr-/- and healthy C57BL/6J control mice have been fixed in 4% neutral formaldehyde, embedded in paraffin and sliced into 4 μm thin sections. One of the sections has been stained with hematoxylin-eosin, the other one with green trichrome. Staining with hematoxylin-eosin is primary staining used for basic imaging of histological structure of tissues, staining with green trichrome is used for imaging...

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1 Němečková, Iveta
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