National Repository of Grey Literature 20 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
Bazotests and lymphocyte transformation tests linked to drug intolerance
Honsárková, Tereza ; Krčmová, Irena (advisor) ; Andrýs, Ctirad (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Science Candidate: Bc. Tereza Honsárková Supervisor: prim. MUDr. Inrena Krčmová, CSc. Title of diploma thesis: Bazotests and lymphocyte transformation tests linked to drug intolerance Basophil activation tests and lymphocyte transformation tests are methods that allow drug allergy diagnosis. The right diagnosis is crucial for treatment. Drug allergy is often associated with β-lactam antibiotics hypersensitivity and especially with penicillins. The aim of this diploma thesis is to provide actual statistics based on selected patient medical reports. Those patients were completely clinically and laboratory examined. The main condition for data selection was the execution of skin tests for suspicion of drug allergy caused by penicillin-like antibiotics at the Institute of Clinical Immunology and Allergology of the Faculty Hospital Hradec Králové in 2017. Patients' anamnesis, skin tests and laboratory examination focused on basophil activation tests and lymphocyte transformation was composed. Statistics of 25 patients shows beneficial informations. Delayed manifestations are the most prevalent (63 %) in patients which is interesting. However the patients with positive skin test on major determinant had acute...
Bazotests and lymphocyte transformation tests linked to drug intolerance
Honsárková, Tereza ; Krčmová, Irena (advisor) ; Andrýs, Ctirad (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Science Candidate: Bc. Tereza Honsárková Supervisor: prim. MUDr. Inrena Krčmová, CSc. Title of diploma thesis: Bazotests and lymphocyte transformation tests linked to drug intolerance Basophil activation tests and lymphocyte transformation tests are methods that allow drug allergy diagnosis. The right diagnosis is crucial for treatment. Drug allergy is often associated with β-lactam antibiotics hypersensitivity and especially with penicillins. The aim of this diploma thesis is to provide actual statistics based on selected patient medical reports. Those patients were completely clinically and laboratory examined. The main condition for data selection was the execution of skin tests for suspicion of drug allergy caused by penicillin-like antibiotics at the Institute of Clinical Immunology and Allergology of the Faculty Hospital Hradec Králové in 2017. Patients' anamnesis, skin tests and laboratory examination focused on basophil activation tests and lymphocyte transformation was composed. Statistics of 25 patients shows beneficial informations. Delayed manifestations are the most prevalent (63 %) in patients which is interesting. However the patients with positive skin test on major determinant had acute...
The sensitivity of C-reactive protein levels after total hip endoprosthesis
Fremundová, Petra ; Jílek, Petr (advisor) ; Andrýs, Ctirad (referee)
C-reactive protein is an acute phase protein and is one of the longest used laboratory methods in the diagnosis of infectious or other inflammatory conditions. Replacement of the hip joint is one of the most common orthopedic surgeries performed on workplaces. One of the most serious complications of these procedures is an infection in the area of compensation. This work deals with the diagnostic possibilities of prevention of infectious complications after replacement of the hip joint using measurements of the levels of C-reactive protein in the postoperative period. On the set of 30 patients after replacement of the hip joint was monitored the development of the values of C- reactive protein at regular intervals in the postoperative period. Levels were set one of the available optical methods and evaluated 1., 3., 5. and 7. of post-operative day. On the basis of the results obtained with the work of trying to evaluate their own sensitivity and specificity of laboratory tests in C - reactive protein in the early postoperative period after the replacement of the hip joint. From the results, it is obvious a significantly higher sensitivity tests than its specificity. At the same time, however, is a clear positive contribution to the examination of the dynamics, in particular in monitoring the levels...
Levels of umbilical cord blood pathogen pattern receptors and proinflammatory molecules - the relation to histological chorioamnionitis
Mitášová, Eva ; Andrýs, Ctirad (advisor) ; Procházka, Martin (referee) ; Ulčová-Gallová, Zdeňka (referee)
The umbilical cord blood (UCB) represents the earliest haematological sample of the fetus/neonate without invasive intervention or any risk for mother and her child during sampling. From the UCB we are able to determine a lot of information about the clinical status of newborn. The fetal response to preterm birth with IAI is characterised by increased levels of proinflammatory cytokines in the UCB. Our aim was therefore to investigate the association of IAI and immunological parameters interleukin IL-6, IL-8, matrix metalloproteinase 8 (MMP-8), pentraxin 3 (PTX3), soluble form of Toll-like receptor 2 (sTLR2) and soluble form of scavenger receptor CD163 (sCD163). The strengths of our study include quantification of sTLR2, sCD163, PTX3 and MMP-8 in UCB and their relationship to IAI that has never been examined. There was a significant increase in inflammatory markers IL-6 and sCD163 in UCB in infants exposed to preterm premature rupture of membranes (PPROM) and to histological chorioamnionitis (HCA) compared to those without intraamniotic inflammation (IAI). There was significant differences in IL-6, IL-8, MMP-8 and sCD163 levels in those with and without funisitis in women with PPROM. However, no significant differences were observed between levels of sTLR2 and PTX3 in UCB in subjects with and...
The effect of Goeckerman therapy on selected markers of angiogenesis and inflammation in psoriasis patients
Pohl, David ; Andrýs, Ctirad (advisor) ; Hernychová, Lenka (referee) ; Petrisková, Jela (referee)
Psoriasis is one of the most common skin diseases affecting about 2-3% of the caucasian population. Its clinical presentation is the formation of red scaled plaques on the skin. The main pathological features of psoriatic plaque comprise of skin inflammation, disturbances of keratinocyte proliferation and maturation and angiogenesis. Although the precise precipitating mechanism of psoriasis has not been discovered yet, the key element in psoriasis initiation is T- lymphocyte population. Nevertheless, other inflammatory cells (e.g. neutrophils, macrophages) as well as activated endothelium play a role in its pathogenesis. Indeed, one of the first histological changes in the affected skin is leukocyte infiltration. The so called trafficking of inflammatory cells to the skin is tightly regulated process guided by cytokines especially chemokines. Angiogenesis is a process of new blood vessel growth from preexisted vessel bed. Vessels in the most of the tissues of adult individual remain in a state of quiescence. The rate of endothelial cell turnover is relatively slow (1-2 years). However in cases of physiological or pathological requirements, a new vessel formation can be initiated by the mechanism called angiogenic switch. One of the pathological conditions associated with angiogenesis is formation of...
Allergic sensitisation and food allergy ( oral allergic syndrom)
Kmoníčková, Michaela ; Krčmová, Irena (advisor) ; Andrýs, Ctirad (referee)
Kmoníčková M.: Allergic sensitisation and food allergy (oral allergic syndrom). Charles University in Praque, Faculty of Pharmacy in Hradec Králové, Department of Biological and Medical Science, Hradec Králové 2012, 56 p. Food allergy is one of the most spread allergic diseases. Oral allergic syndrome is the most common form of food allergy occurred at adult population which stems from a cross- reactivity between pollen allergens and food allergens of vegetable origin. Nowadays it is known as pollen-food syndrome. There has been laboratory tested 40 patients positive for sIgE by ISAC as a part of this thesis. Results were evaluated in relation to clinical manifestations reported in order to establish a positive relationship between the sIgE and probability of occurrence of clinical manifestation. The most frequent incidence of clinical symptoms have been founded after eating apple in patients with pollen allergy Bet v 1 and Aln g 1. This positive relationship between sIgE and clinical positive manifestations are very tight and in terms if diagnostic benefit. The probability of chance of clinical manifestation at patients who were eating hazelnuts and positive sIgE is 7% and at patients who were eating peaches is the probability of chance of clinical manifestation more than 50%. In the case of...
The effect of Goeckerman therapy on selected immune markers in patients with psoriasis
Kondělková, Kateřina ; Andrýs, Ctirad (advisor) ; Weigl, Evžen (referee) ; Posová, Helena (referee)
1 Summary The effect of Goeckerman therapy on selected immune markers in patients with psoriasis Psoriasis is a systemic inflammatory disorder with skin manifestation. Classical approach to treat psoriasis is Goeckerman therapy (GT) which is based on daily application of pharmaceutical coal tar on affected skin with subsequent exposition to UV light. The aim of this study was to observe selected immunological markers and cell subpopulations in patients with psoriasis and to elucidate the effect of GT on these parameters. We evaluated T regulatory cells (Treg), membrane CD163 expression on monocytes, serum levels of CD163 (sCD163), membrane TLR2 and TLR4 expression on monocytes and serum levels of TLR2 (sTLR2). The immunofluorescent analysis was performed on erythrocyte lysed peripheral blood using a flow cytometer FC500 Cytomics (Beckman Coulter) and FACSCalibur (Becton Dickinson). The level of soluble markers was detected by sandwich enzyme-liked immunosorbent assay (ELISA). There was no significant difference in the relative number of Treg cells in the peripheral blood of healthy blood donors and patients with psoriasis before initiation of GT. The relative number of Treg is significantly increased in psoriatic patients after GT compared to the pre-treatment level. In comparison with levels in controls...
Cholesterol depletion using reopheretic and apheretic processes and impact of these methods on immune system
Svrčková, Ellen ; Andrýs, Ctirad (advisor) ; Krejsek, Jan (referee)
LDL-apheresis and haemorheopheresis are the most frequent methods of extracorporeal ellimination methods used for lowering the LDL cholesterol in patients with familial hypercholesterolaemia (FH). In case of failure of conservative therapy (represented by pharmacotherapy and/or dietary regime) these methods reprensent the effective and accessible solution for the clinical status characterized by high morbidity and mortality as well. The lipid components are the most frequent observed markers for the effeciveness of intervention. However, immunity with its effector molecules plays also essential role and there is suppose, that could also reflect the state and progress of atherosclerosis in FH patients. The aim of this thesis was to evaluate the levels of selected immunological markers (plasmatic glykoprotein α-2-macroglobulin, IL-10, soluble endoglin, soluble apoptotic factor sAPO-Fas and soluble form of adhesive P-selectin) and their changes after LDL-apheresis and haemorheopheresis employing enzyme immunoanalysis and immuno nephelometry. Totally, 12 patients were involved in this study, 3 were treated by haemorheopheresis, the rest 9 received LDL-apheresis. As a results, significant decreases in serum levels of α-2- macroglobulin, soluble endoglin and sAPO-1/Fas were recorded. Observed changes of...

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