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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Formation of blastema during limb regeneration in Amphibia
 Paušlyová, Lucia ; Tlapáková, Tereza (advisor) ; Paňková, Daniela (referee)
Total limb regeneration among vertebrates is basically restricted to some amphibians. Urodeles have the ability to regenerate amputated limbs through their life span. Anurans have the ability of complete regeneration of amputated limbs only in their larval stage. The key process of the limb regeneration is the formation of undifferentiated cell group which is called blastema. There are many cell types that contribute to formation of the blastema while the most important part in this process belongs to the skeleton muscle tissue and dermal fibroblasts. Another critical factor in formation of the blastema and its growth are the nerves in the area of wound and neurotrophic factors produced by them. In the last 20 years it has been great improvement in using different markers for tracking the fate of blastema cells.
Detailed record
Formation of blastema during limb regeneration in Amphibia
 Paušlyová, Lucia ; Tlapáková, Tereza (advisor) ; Paňková, Daniela (referee)
Total limb regeneration among vertebrates is basically restricted to some amphibians. Urodeles have the ability to regenerate amputated limbs through their life span. Anurans have the ability of complete regeneration of amputated limbs only in their larval stage. The key process of the limb regeneration is the formation of undifferentiated cell group which is called blastema. There are many cell types that contribute to formation of the blastema while the most important part in this process belongs to the skeleton muscle tissue and dermal fibroblasts. Another critical factor in formation of the blastema and its growth are the nerves in the area of wound and neurotrophic factors produced by them. In the last 20 years it has been great improvement in using different markers for tracking the fate of blastema cells.
Detailed record
Transport 2,4 - dichlorfenoxyoctové kyseliny v explantátech okurky seté (Cucumis sativus L.)
 Hajdamachová, Iva
For the experiment were used threecentimeters hypocotyl segments cucumbers sown (Cucumis sativus L.), cultured on MS medium and induction medium with added growth regulators. The aim was to demonstrate bazipetal transport 2,4- dichlorophenoxyacetic acid (2,4 - D ) in hypocotyls . Transport 2,4-D was detected by morphological evaluation of all variants of the experiment, by determining the weight of dry matter and distribution of 14C-activity after uptake 14C-2,4-D from the culture medium. 2,4-D induced callus in hypocotyls cultured on induction medium and subcultured in hypocotyls after 3 and 6 days on MS medium . The largest callus formed at the basal part of hypocotyls, which confirmed bazipetal transport 2,4-D. During cultivation hypocotyls were found bazipetal distribution of 14C-activity originating 14C-2,4-D. The hypocotyls cultured on MS medium was successfully formed roots on theirs basal parts, but for variants subcultured on MS medium for 3 days, the resulting larger roots.
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