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Importance of biosensors and biomarkers for environment contamination assessment
Mišo, Milan ; Čáslavský, Josef (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis is purely theoretical. It aims to explain the principle and nature of operation of biosensors and biomarkers, their properties, distribution and use in the assessment of environmental contamination. It describes their practical use. In the first part of the thesis are described biosensors. Biosensors combine biorecognition molecules and a suitable converter, this combination allows you to convert (bio)chemical information into electrical signals that can be easily measured and therefore are becoming more used for determination of different substances. The second section describes the early warning signs called biomarkers. Biomarkers provide information on changes in the ecosystem before it is irreversible damaged, and we can intervene early.
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Designing and testing of new metal nanosubstrates for biomolecular sensors based on surface-enhanced Raman scattering (SERS) spectroscopy
Peksa, Vlastimil ; Procházka, Marek (advisor) ; Matějka, Pavel (referee) ; Richter, Ivan (referee)
Title: Designing and testing of new metal nanosubstrates for biomolecular sensors based on surface-enhanced Raman scattering (SERS) spectroscopy Author: Vlastimil Peksa Department: Institute of Physics of Charles University Supervisor: doc. RNDr. Marek Procházka, Ph.D., Institute of Physics of Charles University Abstract: This experimental methodical work was aimed at the optimization of selected gold and silver substrates and their use in construction of SERS-based biosensors, including following practical application. Several types of substrates, fabricated via a combination of bottom-up techniques on solid surfaces, were tested. The properties of these substrates were examined with probe molecules, namely methylene blue, porphyrins and tryptophan, on a confocal Raman microspectrometer. Obtained findings about the influence of analyte application, objective focusing and internal intensity standard were exploited for optimization of measurement procedures with regard to sensitivity, accuracy and reproducibility. A method for quantitative detection of food dye azorubine (E 122) in commercially available drinks was developed, based on these findings. Its results have shown its potential as a pre-scan method for field application and preliminary testing. Keywords: Metal nanosubstrates, biomolecules,...
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Importance of biosensors and biomarkers for environment contamination assessment
Mišo, Milan ; Čáslavský, Josef (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis is purely theoretical. It aims to explain the principle and nature of operation of biosensors and biomarkers, their properties, distribution and use in the assessment of environmental contamination. It describes their practical use. In the first part of the thesis are described biosensors. Biosensors combine biorecognition molecules and a suitable converter, this combination allows you to convert (bio)chemical information into electrical signals that can be easily measured and therefore are becoming more used for determination of different substances. The second section describes the early warning signs called biomarkers. Biomarkers provide information on changes in the ecosystem before it is irreversible damaged, and we can intervene early.
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