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Microrheology of Biocolloids
Hnyluchová, Zuzana ; Omelka, Ladislav (referee) ; Kráčalík, Milan (referee) ; Pekař, Miloslav (advisor)
The main aim of the doctoral thesis was study of passive microrheological techniques as advanced methods for characterisation of viscoelastic properties of soft material. These techniques are able to provide certain advantaged or additional information on classical rheology. Several techniques from the wide range of passive microrheological group such as one particle video – microrhelogy with IDL data procesing, microrheology based on light scattering – DLS or FCS microrheology have been applied in practice during my PhD studies. New Matlab script has been also invented to simply obtain information about viscosity of low volume samples. Aplicability of techniques were studied on several various bicolloids such as solutions or gels of hyaluronic acid, on study of properties at the interfaces or on gelation proces studies of agarose. Obtained data were compared classical rheology results. Suitability of each technique for investigated systems or appropriate reccomendations for further measurements were didcussed based on obtained data. Main advantages and limites of passive microrheology techniques were also described in comparison wih classical rheology method.
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Study of hydration of biocolloids
Hývnarová, Lucie ; Sedláček, Petr (referee) ; Klučáková, Martina (advisor)
The subject of this master’s thesis was to examine the hydration properties of selected biocolloids. Specifically, humic substances (fulvic and humic acids), hyaluronic acids in three molecular weights, dextran and chitosan were investigated. Hydration properties of selected biocolloid substances were studied by various methods. The influence of water content during the dissolution of substances and the different time for which water acted on biocolloids were studied. Differential scanning calorimetry DSC measurements resulted in enthalpies and crystallization and melting points. Heat fluxes resulted in the use of perfusion calorimetry. TGA thermogravimetric analysis was used to determine the moisture content of the bioolloids used, after measuring the perfusion calorimeter.
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New Methods of Study of Reactivity and Transport Properties of Biocolloids
Smilek, Jiří ; Weidlich,, Tomáš (referee) ; Pabst, Willi (referee) ; Klučáková, Martina (advisor)
The main aim of doctoral thesis was the study on reactivity, transport and barrier properties of biocolloidal and synthetic polymeric substances by simple diffusion techniques. It was studied mainly the influence of basic physic-chemical parameters (temperature, concentration, pH and modification of material) on the reactivity and barrier ability of chosen compounds. Further substances were chosen as a model compounds: biocolloids (humic acids, alginate, chitosan, hyaluronate) and synthetic polymer (polystyrenesulfonate). Reactivity, barrier and transport properties of chosen substances were studied by interactions with oppositely charged basic organic dyes (methylene blue, rhodamine 6G, amido black 10B respectively) in hydrogels medium based on linear polysaccharide (agarose). The attention was also paid to basic physic-chemical characterisation (infrared spectroscopy, rheology, elemental analysis, thermogravimetry and scanning electron microscopy) of chosen materials and also hydrogels. Key part of the whole doctoral thesis was the optimization of selected diffusion techniques (diffusion cell technique and non-stationary diffusion in cuvettes) designated for the study on reactivity and barrier properties of wide range compounds (optimized method should be used as an universal method for simple and fast determination of reactivity of different compounds at given or changing conditions). The rate of reactivity, transport and barrier properties was determined based on fundamental diffusion parameters such as diffusion coefficients, break-through time so called lag time, interfacial concentration of chosen organic dye, apparent equilibrium constant, tortuosity factor, partition coefficient.
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Dynamic tensiometry of biocolloids
Kulilová, Pavlína ; Mašek, David (referee) ; Pekař, Miloslav (advisor)
Hyaluronová kyselina je v současné době velmi významná biomolekula používaná v medicíně a kosmetice, a její výzkum je důležitý pro další budoucí použití. Zaměření této práce je studování povrchových vlastností hyaluronové kyseliny, jejích hydrofobních derivátů a roztoků SDS pro srovnání, za použití tenziometrie. Tyto sloučeniny byly zkoumány ve vodě a v roztocích ve formě sodné soli. Sledované vzorky byly měřeny dvěmi metodami v různých koncentračních rozmezích při laboratorní teplotě pomocí nového BPA-800P bublinového tenziometeru. Byly navrženy takové experimenty, aby se zjistily využitelné možnosti tohoto přístroje pro další výzkum. Výsledky práce ukazují rozdílnosti jednotlivých povrchově aktivních látek v závislosti na jejich koncentraci a použitém prostředí. Hyaluronová kyselina nevykazuje povrchovou aktivitu, zatímco její deriváty a SDS ano.
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Hydration of biocolloids - calorimetric study
Šméralová, Ester ; Pekař, Miloslav (referee) ; Klučáková, Martina (advisor)
Presented master's thesis focuses on the study of hydration of selected biocolloid substances, specifically humic substances (humic acids and fulvic acids), hyaluronic acid with three different molecular weight, chitosan and dextran. Interaction of biocolloids with water was studied by different methods. The effect of solubility, structure, functional groups in molecule on sorption and hydration ability of these biocolloids was investigated. In the case of hyaluronan the influence of molecular weight was also study. Differential scanning calorimetry DSC and perfusion calorimetry give results of heat of hydration, enthalpies and temperature of crystallization and melting. Thermogravimetric analysis TGA was used to determine the original moisture content of the samples.
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Study of hydration of biocolloids
Hývnarová, Lucie ; Sedláček, Petr (referee) ; Klučáková, Martina (advisor)
The subject of this master’s thesis was to examine the hydration properties of selected biocolloids. Specifically, humic substances (fulvic and humic acids), hyaluronic acids in three molecular weights, dextran and chitosan were investigated. Hydration properties of selected biocolloid substances were studied by various methods. The influence of water content during the dissolution of substances and the different time for which water acted on biocolloids were studied. Differential scanning calorimetry DSC measurements resulted in enthalpies and crystallization and melting points. Heat fluxes resulted in the use of perfusion calorimetry. TGA thermogravimetric analysis was used to determine the moisture content of the bioolloids used, after measuring the perfusion calorimeter.
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Hydration of biocolloids - calorimetric study
Šméralová, Ester ; Pekař, Miloslav (referee) ; Klučáková, Martina (advisor)
Presented master's thesis focuses on the study of hydration of selected biocolloid substances, specifically humic substances (humic acids and fulvic acids), hyaluronic acid with three different molecular weight, chitosan and dextran. Interaction of biocolloids with water was studied by different methods. The effect of solubility, structure, functional groups in molecule on sorption and hydration ability of these biocolloids was investigated. In the case of hyaluronan the influence of molecular weight was also study. Differential scanning calorimetry DSC and perfusion calorimetry give results of heat of hydration, enthalpies and temperature of crystallization and melting. Thermogravimetric analysis TGA was used to determine the original moisture content of the samples.
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Microrheology of Biocolloids
Hnyluchová, Zuzana ; Omelka, Ladislav (referee) ; Kráčalík, Milan (referee) ; Pekař, Miloslav (advisor)
The main aim of the doctoral thesis was study of passive microrheological techniques as advanced methods for characterisation of viscoelastic properties of soft material. These techniques are able to provide certain advantaged or additional information on classical rheology. Several techniques from the wide range of passive microrheological group such as one particle video – microrhelogy with IDL data procesing, microrheology based on light scattering – DLS or FCS microrheology have been applied in practice during my PhD studies. New Matlab script has been also invented to simply obtain information about viscosity of low volume samples. Aplicability of techniques were studied on several various bicolloids such as solutions or gels of hyaluronic acid, on study of properties at the interfaces or on gelation proces studies of agarose. Obtained data were compared classical rheology results. Suitability of each technique for investigated systems or appropriate reccomendations for further measurements were didcussed based on obtained data. Main advantages and limites of passive microrheology techniques were also described in comparison wih classical rheology method.
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New Methods of Study of Reactivity and Transport Properties of Biocolloids
Smilek, Jiří ; Weidlich,, Tomáš (referee) ; Pabst, Willi (referee) ; Klučáková, Martina (advisor)
The main aim of doctoral thesis was the study on reactivity, transport and barrier properties of biocolloidal and synthetic polymeric substances by simple diffusion techniques. It was studied mainly the influence of basic physic-chemical parameters (temperature, concentration, pH and modification of material) on the reactivity and barrier ability of chosen compounds. Further substances were chosen as a model compounds: biocolloids (humic acids, alginate, chitosan, hyaluronate) and synthetic polymer (polystyrenesulfonate). Reactivity, barrier and transport properties of chosen substances were studied by interactions with oppositely charged basic organic dyes (methylene blue, rhodamine 6G, amido black 10B respectively) in hydrogels medium based on linear polysaccharide (agarose). The attention was also paid to basic physic-chemical characterisation (infrared spectroscopy, rheology, elemental analysis, thermogravimetry and scanning electron microscopy) of chosen materials and also hydrogels. Key part of the whole doctoral thesis was the optimization of selected diffusion techniques (diffusion cell technique and non-stationary diffusion in cuvettes) designated for the study on reactivity and barrier properties of wide range compounds (optimized method should be used as an universal method for simple and fast determination of reactivity of different compounds at given or changing conditions). The rate of reactivity, transport and barrier properties was determined based on fundamental diffusion parameters such as diffusion coefficients, break-through time so called lag time, interfacial concentration of chosen organic dye, apparent equilibrium constant, tortuosity factor, partition coefficient.
|
|
|
Dynamic tensiometry of biocolloids
Kulilová, Pavlína ; Mašek, David (referee) ; Pekař, Miloslav (advisor)
Hyaluronová kyselina je v současné době velmi významná biomolekula používaná v medicíně a kosmetice, a její výzkum je důležitý pro další budoucí použití. Zaměření této práce je studování povrchových vlastností hyaluronové kyseliny, jejích hydrofobních derivátů a roztoků SDS pro srovnání, za použití tenziometrie. Tyto sloučeniny byly zkoumány ve vodě a v roztocích ve formě sodné soli. Sledované vzorky byly měřeny dvěmi metodami v různých koncentračních rozmezích při laboratorní teplotě pomocí nového BPA-800P bublinového tenziometeru. Byly navrženy takové experimenty, aby se zjistily využitelné možnosti tohoto přístroje pro další výzkum. Výsledky práce ukazují rozdílnosti jednotlivých povrchově aktivních látek v závislosti na jejich koncentraci a použitém prostředí. Hyaluronová kyselina nevykazuje povrchovou aktivitu, zatímco její deriváty a SDS ano.
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