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National Repository of Grey Literature

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NMR Metabolomics in Toxicology: Effect of NPs Inhalation on Blood Plasma and Exhaled Breath Condensate Profiles. Michálková, Lenka In our study, 1H NMR metabolomics was employed to analyse samples of exhaled breath condensate and blood plasma of control subjects and workers before and after the shift where various tasks related to the processing of nanocomposite materials were performed. The main aim of this work is to evaluate the molecular changes induced by NPs inhalation. Plný tet: PDF Detailed record

Metabolomics Study of the Association between Pancreatic Cancer and Diabetes Mellitus. Michálková, Lenka ; Horník, Štěpán ; Sýkora, Jan ; Habartová, L. ; Setnička, V. In our study, 1H NMR metabolomics was employed to plasma samples of pancreatic cancer patients, individuals with long-term diabetes mellitus type 2 (lasting more than 5 years) and healthy controls. The NMR analyses were followed by establishing a statistical model based on principal component analysis and discriminant analysis. The aim was to discover differences between these groups and to define a potential biomarker panel. The statistical evaluation of metabolomics-based profiles provided high values of sensitivity and specificity. Subsequently, plasma samples of the risk group, specifically patients with recently diagnosed diabetes mellitus with a duration of <3 years (possible T3cDM), were analysed and the possibility of PC development was predicted. The achieved results showed strong potential of 1H NMR metabolomics to establish a biomarker panel that would facilitate the early diagnosis of PC and the possibility identify diabetic individuals, who are at risk of developing PC. Fulltext: content.csg - PDF Plný tet: SKMBT_C22019041212340 - PDF Detailed record

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