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Efekt selenu na antioxidační status organismu po porodu
Wronková, Sabina
The aim of this thesis was to develop a literature search and experimentally determine the effect of organic forms of selenium levels (0.3 and 0.6 mg/kg FM) of selenium on the antioxidant status in postpartum sows. Experiment included 18 sows in the postpartum period, which were divided into three groups according to age. The model animals were divided into three groups. The first group (Se1) sows (n = 6) served as a control, wherein the addition of selenium has not been increased. The second group (Se2) sows (n = 6) were fed 0.3 mg Se/kg FM. Third group (Se3) sows (n = 6) were fed 0.6 mg Se/kg FM. Premix was dosed in the morning feeding. To supplement selenium were used selenium enriched yeast (Sel-Plex - Alltech's). The results show that the dose of selenium in an amount of 0.30 and 0.60 mg/kg FM, increase the activity of antioxidant enzymes, glutathione peroxidase and superoxide dismutase. Glutathione peroxidase (GPx) after supplementation with 0.3 mg/kg selenium increased from the second sampling, but the third sampling was statistically significant (P <0.05). Doses of 0.6 mg/kg selenium also visibly increased level of glutathione, but these results can not be statistically demonstrated. Superoxide dismutase (SOD) at both doses (0.3 and 0.6 mg/kg) appeared selenium statistically not detectable visible growth. We also found an increase of antioxidant activity measured by the methods ABTS, FRAP and FR, although the methods ABTS and FR showed slight fluctuations. The amount of reduced and oxidized glutathione were also significantly (P <0.05) higher in both experimental groups. The selenium content of selenium supplementation significantly affected when the results of both experimental groups were statistically significant (P <0.05) higher. Level malonyldialdehydu (MDA) after supplementation with 0.3 mg/kg selenium statistically proven (P <0.05) increased in the third collection at 0.6 mg/kg selenium, we observed statistically demonstrable (P <0.05) increase from the second collection. We can therefore conclude that selenium supplementation in organic form, can reduce oxidative stress in confinement of sows and overcome this stressful period for the organism.
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Efekt selenu na antioxidační status organismu po porodu
Wronková, Sabina
The aim of this thesis was to develop a literature search and experimentally determine the effect of organic forms of selenium levels (0.3 and 0.6 mg/kg FM) of selenium on the antioxidant status in postpartum sows. Experiment included 18 sows in the postpartum period, which were divided into three groups according to age. The model animals were divided into three groups. The first group (Se1) sows (n = 6) served as a control, wherein the addition of selenium has not been increased. The second group (Se2) sows (n = 6) were fed 0.3 mg Se/kg FM. Third group (Se3) sows (n = 6) were fed 0.6 mg Se/kg FM. Premix was dosed in the morning feeding. To supplement selenium were used selenium enriched yeast (Sel-Plex - Alltech's). The results show that the dose of selenium in an amount of 0.30 and 0.60 mg/kg FM, increase the activity of antioxidant enzymes, glutathione peroxidase and superoxide dismutase. Glutathione peroxidase (GPx) after supplementation with 0.3 mg/kg selenium increased from the second sampling, but the third sampling was statistically significant (P <0.05). Doses of 0.6 mg/kg selenium also visibly increased level of glutathione, but these results can not be statistically demonstrated. Superoxide dismutase (SOD) at both doses (0.3 and 0.6 mg/kg) appeared selenium statistically not detectable visible growth. We also found an increase of antioxidant activity measured by the methods ABTS, FRAP and FR, although the methods ABTS and FR showed slight fluctuations. The amount of reduced and oxidized glutathione were also significantly (P <0.05) higher in both experimental groups. The selenium content of selenium supplementation significantly affected when the results of both experimental groups were statistically significant (P <0.05) higher. Level malonyldialdehydu (MDA) after supplementation with 0.3 mg/kg selenium statistically proven (P <0.05) increased in the third collection at 0.6 mg/kg selenium, we observed statistically demonstrable (P <0.05) increase from the second collection. We can therefore conclude that selenium supplementation in organic form, can reduce oxidative stress in confinement of sows and overcome this stressful period for the organism.
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