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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
 Vykydalová, Marie ; Duša, Filip ; Horká, Marie ; Šlais, Karel
We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
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Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice
 Vykydalová, Marie ; Horká, Marie ; Šalplachta, Jiří ; Růžička, E. ; Kahle, Vladislav
In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories.
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Differentiation of Candida strains by capillary HPLC
 Vykydalová, Marie ; Moravcová, Dana ; Horká, Marie ; Růžička, F. ; Kahle, Vladislav
The aim of this study was to compare chromatographic profiles of lysate of Candida parapsilosis positive and Candida parapsilosis negative using capilary high performance liquid chromatography.
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4 Vykydalová, Markéta
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