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National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
LightCycler 480 for mtDNA analysis and its use in forensic genetics.
 Voráčková, Lucie ; Klusoňová, Hana (advisor) ; Doleželová, Eva (referee)
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Detekce bakterií rodu Xanthomonas patogenních pro rajče a papriku metodou loop-mediated isothermal amplification
 VORÁČKOVÁ, Lucie
The subject of this thesis is the design of specific LAMP primers and LAMP analysis and its optimization. The purpose of this analysis is to specifically distinguish Xanthomonas gardneri from other pathogenic xanthomonads. Two sets of LAMP primers from the DNA sequences of hrpB and atpD genes were designed. These LAMP primer sets are highly sensitive and the detection limit of LAMP assay was found to be 0.01 l / mg of DNA. The reaction temperature of the LAMP was optimized to 64 °C to reach the maximum of amplification. LAMP can be applied to detection and identification on pathogens in plant tissues. LAMP takes less time than conventional PCR to detect bacteria. This LAMP assay has a great potintial to be applied to detection and identification of Xanthomonas genus bacteria pathogenic for tomato and pepper.
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LightCycler 480 for mtDNA analysis and its use in forensic genetics.
 Voráčková, Lucie ; Klusoňová, Hana (advisor) ; Doleželová, Eva (referee)
Detailed record
DNA čipy a jejich využití pro molekulární diagnostiku a predikci u onkologických onemocnění
 VORÁČKOVÁ, Lucie
Many visible factors produced during carcinogenesis refer to oncological disease, e.g. modified gene's expression or increased number of miRNA. These factors can be diagnosed by analyzing of gene's expression. For this diagnostics, there are used many methods, of whom DNA microarray analysis is the most significant progress in genetic research. A large number of DNA sequences can be detected DNA with microarrays on a single microarray in a relatively short time. This analysis means a positive turnover for the diagnosis and prediction of disease.
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See also: similar author names
3 VORÁČKOVÁ, Lenka
5 VORÁČKOVÁ, Lucie
3 Voráčková, Lenka
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