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National Repository of Grey Literature

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Optimization of liver spheroids preparation Vojtěchová, Tereza ; Boušová, Iva (advisor) ; Szotáková, Barbora (referee) Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Tereza Vojtěchová Supervisor: assoc. prof. PharmDr. Iva Boušová, Ph.D. Advisor: PharmDr. Martin Ambrož, Ph.D. Title of diploma thesis: Optimization of liver spheroids preparation Various in vitro liver models play an irreplaceable role in preclinical drug evaluation. One of them is multicellular hepatic spheroids. These are 3D cell aggregates that are formed by the process of cellular aggregation. They are used in drug development and represent a promising tool for identifying new targets and drug screening for various liver diseases. The aim of this thesis was to introduce and optimize the preparation of liver spheroids by the "forced-floating" method. The liver of male and female Wistar rats was used for hepatocyte isolation and spheroid preparation. Due to the low viability of isolated hepatocytes, we tried to optimize the isolation procedure. Nevertheless, we managed to obtain only a small number of hepatocytes with sufficient viability for subsequent formation of spheroids. The spheroids were incubated with various lipogenic reagents for 7 and 14 days and their viability was monitored by measuring adenosine triphosphate (ATP) production. Incubation of spheroids with lipogenic substrates... Detailed record

Using of the comet assay in clinical practice Vojtěchová, Tereza ; Kuchařová, Monika (advisor) ; Bárta, Pavel (referee) Detailed record

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