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The Modulus of Elasticity of Air-Entrained and Non-Air-Entrained Concrete
Tichý, Aleš ; Misák, Petr (referee) ; Kocáb, Dalibor (advisor)
The bachelor thesis deals with the verification of the degree of influence of the air-entraining admixture on the modulus of elasticity of concrete. The experiment was carried out for these purposes. The values of static and dynamic modulus of elasticity were determined on drilled cores from concrete blocks of different mixtures. The magnitudes of dynamic modulus of elasticity were determined by using an ultrasonic pulse velocity method and a resonance method. The results are summarized in tabular and graphical form.
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Heme sensor proteins as potential biomarkers of cellular and oxidative stress processes induced by ionizing radiation
Vávra, Jakub ; Martínková, Markéta (advisor) ; Souček, Pavel (referee) ; Tichý, Aleš (referee)
[IN CZECH] Ionizing radiation is a potential inducer of the oxidative stress processes in cells. As a result, reactive radicals are formed in the intracellular space modifying the essential biomolecules. Ionizing radiation has either direct (radiation sickness) or indirect (malignant processes) effect on the organism. Therefore, a fast determination of the dose is required when suspected irradiation of the organism occurs. However, a method routinely applicable, fast enough and at the same time suitable for dose estimation based on biomarkers has not been developed so far. The aim of this thesis is to describe new properties of the selected heme sensor proteins and discuss their potential importance for the cellular adaptation to oxidative stress conditions. Specifically, the thesis is focused on two eukaryotic proteins, heme regulated inhibitor (HRI) and transcription factor p53. The study of functional regulation as well as the conformational changes of these proteins induced by heme is greatly emphasized. Besides, the optimization of the key experimental methods was conducted. Specifically, Phos-tag electrophoresis was applied for the kinetics study of HRI wild type and its Gly202Ser mutant form, which is a characteristics of lung cancer development. Unsurprisingly, for both HRI forms studied,...
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Analysis of flagellar proteins in Clostridium difficile isolates of clinically relevant PCR-ribotypes
Houdková, Kateřina ; Konečná, Klára (advisor) ; Tichý, Aleš (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Houdková MPharm Supervisor: RNDr. Klára Konečná, Ph.D. Consultant: PharmDr. Jiří Dresler, Ph.D. Title of rigorous thesis: Analysis of flagellar proteins in C. difficile isolates of clinically relevant PCR-ribotypes Background: Strains of C. difficile of known human epidemiologic importance are associated with severe clinical features of C. difficile infection (CDI). In this study, a panel of eight different PCR-ribotypes (RTs) with their proteins released in vitro were subjected to analysis. The aim of this work is to monitor the relationship between secretions of individual proteins associated with flagellar formation and function in C. difficile strains of variable virulence. Methods: Within our research, a combination of tandem mass spectrometry with liquid chromatography was used. The semi-quantitative analysis employed label free quantification (LFQ) approach. Results: From the quantifiable proteins, 17 were significantly increased in functional annotations. Among them, several known factors connected with flagellar assembly and other functions were identified. Higher expression of selected flagellar proteins clearly distinguished RTs 027, 176, 005 and 012, confirming the pathogenic...
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Laboratory diagnostics of kidney injury in patients with diabates mellitus I and II.
Múčková, Ľubica ; Dršata, Jaroslav (advisor) ; Tichý, Aleš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Ľubica Múčková Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Consultant: Ing. Jana Netriová, PhD. Title of diploma thesis: Laboratory diagnostics of kidney injury in patients with diabetes mellitus I and II Diabetes mellitus is a chronic metabolic disease. This disease leads to develompement of both microvascular and macrovascular complications. In this work we examined a microvascular complication - diabetic nephropathy - and its laboratory diagnostics. We focussed on parameters cystatin C and creatinine. We estimated a glomerular filtration with Grubb, Stevens and MDRD equations. The Grubb and Stevens equations use cystatin C to determine the glomerular fitrlation rate (GFR), MDRD equations use creatinine, albumin and urea. For the asessment of kidney function we evaluated albumin and total protein waste in urine samples collected for 24 hours. The parameters were measured in file of 183 patients. Number of pacients with diabetes I.type was 49 and with diabetes II.type was 134. The main intention of this work was to evaluate equations used for determination of GFR, to compare their pros and cons and put them in contrast with albumin and total proteins waste. In case of the patients...
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In Vitro Study of Anticancer Effect of Amaryllidaceae Montanine Alkaloids
Koutová, Darja ; Havelek, Radim (advisor) ; Tichý, Aleš (referee) ; Chlebek, Jakub (referee)
The possibilities of current therapy of cancer diseases are wide, yet these diseases are still among the most common causes of death. The current findings of biomedical research are increasingly being applied in the treatment strategy of cancer. Understanding the molecular pathways associated with cancer cell formation, progression, and metastasis has led to the discovery of new anticancer drugs and the development of targeted therapies. Simultaneously, knowledge of the molecular mechanism of action of anticancer drugs derived from plants or their semisynthetic derivatives makes it possible to expand the portfolio of cytostatics used so far. The aim of the dissertation was to study the cytotoxic effect of hitherto unexplored montanine-type isoquinoline alkaloids in vitro using a panel of cancer cell lines and a non- cancer cell line of human lung fibroblasts. In the initial phase of the study, the basic types of montanine alkaloids, montanine and pancracine, were isolated, further subjected to cytotoxicity screening using a panel of 9 cancer cell lines of different histotype and one non-cancerous cell line formed by lung fibroblasts. Mean inhibitory concentrations IC50 values were determined. Pancracine, the parent alkaloid of the Amaryllidaceae family, has been comprehensively studied in order to...
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Radiosensitization of cancer cells by modulation of autophagy: Phosphoproteomic analysis
Ondrej, Martin ; Tichý, Aleš (advisor) ; Dresler, Jiří (referee) ; Hernychová, Lenka (referee)
(ENG) Lung cancer, and in particular, non-small cell lung cancer (NSCLC), belongs to the most lethal oncological diseases worldwide. Choosing the proper therapeutic approach is, therefore, inevitable for the successful treatment of lung tumors. In this regard, radiotherapy is an essential therapeutic approach. However, like any other treatment option, radiotherapy has its limitations in the form of radioresistance. Autophagy has been generally accepted to be one of the most crucial factors in the radioresistance of tumor cells. It is, therefore, necessary to search for suitable approaches that would lead to the suppression of cytoprotective autophagy and, thus, to the radiosensitization of tumor cells. In this respect, in our study, we employed methods of quantitative phosphoproteomics along with several techniques of molecular biology to examine the underlying molecular mechanisms and signaling pathways involved in the regulation and inhibition of the autophagic process. To inhibit the cytoprotective autophagy, we used a relatively new autophagy inhibitor-Lys05, the analog of a well-described autophagy inhibitor chloroquine. As a model, we chose the radioresistant NSCLC cells (H1299; p53-negative), because they represent the most problematic group of cells within non-small cell lung tumors. The...
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The Modulus of Elasticity of Air-Entrained and Non-Air-Entrained Concrete
Tichý, Aleš ; Misák, Petr (referee) ; Kocáb, Dalibor (advisor)
The bachelor thesis deals with the verification of the degree of influence of the air-entraining admixture on the modulus of elasticity of concrete. The experiment was carried out for these purposes. The values of static and dynamic modulus of elasticity were determined on drilled cores from concrete blocks of different mixtures. The magnitudes of dynamic modulus of elasticity were determined by using an ultrasonic pulse velocity method and a resonance method. The results are summarized in tabular and graphical form.
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