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Detection of toxic cyanobacteria in Estonian large lakes and coastal waters
TKADLECOVÁ, Anna
This master thesis deals with the detection of toxic cyanobacteria in Estonian lakes (Peipsi, Lämmijärv and Vortsjärv) and coastal waters. Using the molecular qPCR method, the mcyE gene encoding the microcystin toxin (MC) was detected in 3 potential producers - Microcystis, Dolichospermum and Planktothrix. Toxin analysis was performed by LCMS/MS, the results were compared with results of qPCR and statistically evaluated. A positive correlation was found between the concentration of the mcyE gene and the detected MCs. The highest measured values of the occurrence of the mcyE gene were detected in August, with slight deviations depending on the detected organism and the environmental conditions of the lake. The highest occurrence of MCs in the studied waters was detected in September. A positive correlations were also found between the concentration of the mcyE gene, eventual MCs and the amount of cyanobacterial biomass or chlorophyll-a content in water. The results of this work provide an overview of the development of cyanobacteria and their toxins in the Estonian large lakes and in the Baltic Sea during year 2018.

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1 Tkadlecová, Aneta
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