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Compatibility of selected dental alloys with magnetic resonance imaging
Linetskiy, Igor ; Hubálková, Hana (advisor) ; Suchánek, Jakub (referee) ; Dostálová, Taťjana (referee)
Magnetic resonance imaging (MRI) is one of the up-to-date and perspective methods of diagnostic medical imaging. It is highly valued for absence of ionizing radiation, non-invasiveness, acquiring of highly accurate images of hard and especially soft tissues in any plane. It is a method of choice in central nervous system imaging. Nowadays it is used in diagnostics of other diseases in the maxillofacial area. The major shortcoming of MRI is interaction of magnetic fields of MRI system with metallic objects present in human body. As a result, this may lead to their heating, movement, and also to imaging artifacts. Since different metallic constructions (fixed bridges, dental implants, splints, plates, orthodontic appliances, etc.) are often used in the contemporary dentistry, the problem of their compatibility with MRI is relevant. The aim of this study was to validate the compatibility of dental alloys with MRI, classify the materials according to the degree of compatibility and to establish the range of their possible negative impact on quality of MRI in head and neck area. In order to find dental metals and alloys, General Health Insurance Company code- list, catalogues of manufacturers' and Prague dental labs were used. Measurement methods of magnetic susceptibility (MS), electric conductivity...
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Release of bisphenol A and its analogues from dental restorative materials
Tichý, Antonín ; Bradna, Pavel (advisor) ; Izakovičová Hollá, Lydie (referee) ; Suchánek, Jakub (referee)
Bisphenol A (BPA) is an endocrine disruptor that may be released from dental polymeric materials. The aim of this study was to examine the kinetics of the long-term release of BPA and its analogues from restorative materials (composites, hybrid glass ionomer cements) and polycarbonates used in dentistry. Extracts in ethanol and artificial saliva were collected after 1-260 days for restorative materials and after 1-84 days for polycarbonates. All extracts were analyzed by ultra high performance liquid chromatography-tandem mass spectrometry. BPA was detected in all tested materials, while its analogues were not found. The release of BPA from all tested materials was highest during the first day, followed by a significant decrease for all materials and a complete stop for the "BPA-free" composites. Among restorative materials, the highest amounts of BPA were released from composites with BPA-based monomers, followed by hybrid glass ionomer cements. The least BPA was released from "BPA-free" composites. Shorter irradiation time did not significantly affect the total amount of BPA released from restorative materials, but it significantly increased the release of BPA during the first day. The release of BPA from polycarbonates was significantly higher than from restorative materials. Given the current...
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Detection of dental caries by using diffuse reflectance spectroscopy
Charvát, Jindřich ; Himmlová, Lucie (advisor) ; Suchánek, Jakub (referee) ; Bartoňová, Marie (referee)
Tooth decay is one of the most common infectious diseases. Its early detection is important to prevent the progression of the disease, and thus the emergence of a whole range of complications. Modern diagnostic methods such as laser fluorescence (DIAGNOdent) or transillumination (DIAGNOcam) are reliable in caries detection, especially in ex-vivo studies. However, they have their limitations in clinical use and are therefore only recommended as adjuncts and have not become widely used in practice. Diffuse reflection spectroscopy (DRS) is one of the methods of optical spectroscopy and is based on the principle of measuring the interaction of light with biological tissue. DRS is an experimental method for dentistry today. Currently, we are not aware of a study that would investigate the detection of dental caries using the DRS method in greater detail. This work deals with the possibilities of this method in the analysis of hard dental tissues and its use in the detection of dental caries. The study is divided into 5 parts: The first part deals with the capabilities of DRS to compare individual hard dental tissues. The second part deals with the ability to detect tooth surface demineralization caused by acids. The third part of the study is devoted to the differences in spectra between healthy and...
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Effect of Hyaluronic Acid on Dental PulpStem Cells
Schmidt, Jan ; Suchánek, Jakub (advisor) ; Dostálová, Taťjana (referee) ; Poleník, Pavel (referee)
Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics and their combined use in tissue engineering is a promising direction in regenerative medicine. DPSCs are a mesenchymal population of stem cells that are obtained from the soft tissues within the dental pulp cavity. Their advantages are easy availability, high proliferative activity, and wide differentiation potential. HA is a natural extracellular biopolymer occurring across human body tissues, including dental pulp. Due to its biocompatibility and biodegradability, HA is a suitable scaffold materal. Degradation of high molecular weight (HMW) HA chains, which are enzymatically cleaved in tissues, results in low molecular weight (LMW) HA fragments. Compared to the HMW HA, LMW HA exhibits markedly different bioactive properties. Thus, it is necessary to assess the effect of HA on tissues and cells separately for its distinct fractions. The results of two in vitro experiments are presented in this work. The first of them was focused on the influence of a wide spectrum of HA (116 kDa, 540 kDa, 1500 kDa) on two DPSC lines. This pilot experiment verified the basic assumption that cells in the environment of selected molecules adhere, survive, proliferate, maintain their typical phenotype and osteogenic and chondrogenic...
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Effect of Cryopreservation on Stem Cells
Pilbauerová, Nela ; Suchánek, Jakub (advisor) ; Dušková, Jana (referee) ; Bílková, Zuzana (referee)
Effect of cryopreservation on stem cells Summary Background: The topic of this study is the cryopreservation of dental pulp stem cells (DPSCs). Cryopreservation is a process of sustaining the viability of cells and tissues by freezing and storing them at sub-zero temperatures where biochemical reactions do not occur. It eliminates the need to preserve stem cells through long-term cultures, allows the storage of stem cells for potential future clinical use or clinical studies. However, it is necessary to fully understand any adverse effects on cryopreserved cells for full applicability in preclinical and clinical practice. The purpose of this study was to determine the effect of cryopreservation on DPSCs stored for 6 and 12 months using an uncontrolled-rate freezing technique. Methods: We successfully isolated ten dental pulp stem cell lineages from donors aged 13 - 18 years to be able to observe the effect of an uncontrolled rate freezing technique on cell size, viability, proliferation activity, relative telomere length, and differentiation potential. We used 10 % dimethyl sulfoxide (DMSO) as a cryoprotective agent (CPA). Results and conclusion: According to the data obtained, the uncontrolled-rate freezing technique is not technically demanding, and it is sufficient for the successful cryopreservation of...
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Dental pulp stem cells
Suchánek, Jakub ; Koberová Ivančaková, Romana (advisor) ; Kukletová, Martina (referee) ; Hampl, Aleš (referee)
The aim of this dissertation study was to optimize the isolation and long term cultivation protocols for human dental pulp stem cells. The protocols which showed best results were used for cultivation of dental pulp stem cell isolated from exfoliated teeth (SHED). Additional aims were to characterize DPSC and SHED and prove their ability to proliferate over Hayflick's limit and differentiate into mature cell lines (osteoblasts, chondroblasts and adipocytes). In order to find optimal protocols for isolation of dental pulp from tooth, we tried three different approaches. During optimization of cultivation protocol we focused on decreasing amount of fetal calf serum (FCS) from 10 % FCS in cultivation media (most often used in literature) into 2 % and thus get closer to cultivation conditions suitable for clinical usage. We compared DPSC cultivated in three different media (medium with 10 % FCS, 2 % FCS supplemented with growth factors and media with 2 % FCS supplemented with ITS and growth factors). For characterization of DPSC and SHED we used basic biological properties (proliferation activity, viability, morphology), their phenotype and karyotype. The study demonstrated that the best protocol for isolation of dental pulp from tooth was to break the roots and extract the pulp throw this aperture. We...
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