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Forensic SNP identification of trichological material by High Resolution Melting analysis
Strobachová, Joanne ; Stenzl, Vlastimil (advisor) ; Korabečná, Marie (referee)
The analysis of mitochondrial DNA (mtDNA) from shed hairs has gained high importance in forensic casework since telogen hairs are one of the common type of evidence left at the crime scene. This type of evidence very often lack of nuclear DNA (nDNA), so the analysis of mtDNA is the only possibility. The analysis of trichological material lacking nDNA is not commonly done in the forensic practice because of the high price of the regularly used mtDNA analysis method, as well as the time demandingness. For these reasons we decided to analyze the mtDNA and identify the trichological material by analysis of chosen single based polymorphism's (SNP) followed by High Resolution Melting (HRM). In order to verify the robustness of the HRM analysis method on several types of biological material that belongs to one person, we tested trichological material in anagen and telogen. From total number of 5 selected SNP's, this paper was able to optimize analysis of 3 SNP's. Due to the analysis of SNP C14766T (applicable for the trichological material in anagen and telogen), SNPs C7028T and G3010A (applicable only for the trichological material in anagen). The mtDNA sequences of different biological materials did not show intra-individual differences. Protocol that could be used for the forensic practice with the use...
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Forensic SNP identification of trichological material by High Resolution Melting analysis
Strobachová, Joanne ; Stenzl, Vlastimil (advisor) ; Korabečná, Marie (referee)
The analysis of mitochondrial DNA (mtDNA) from shed hairs has gained high importance in forensic casework since telogen hairs are one of the common type of evidence left at the crime scene. This type of evidence very often lack of nuclear DNA (nDNA), so the identification based on the STR analysis is not possible. The analysis of trichological material lacking nDNA is not a commonly used method of the forensic praxis because of the high price of the regularly used mtDNA analysis method, as well as the time demandingness. Based on this, the main goal of this work is to establish a relatively fast, easy and cheap method able to analyze trichological material lacking of nDNA. For these reasons we decided to analyze the mtDNA and identify the trichological material by analysis of chosen SNP's followed by High Resolution Melting. We have analyzed 12 series of different biological materials that belongs to one person (e.g. buccal cells, trichological material in anagen - head, axillary and pubic hair shafts) in order to confirm the identity of mtDNA isolated from different type of biological materials. We have analyzed several set's of buccal cells and head hair shafts in telogen from 17 specimens. From total number of 5 selected SNP's, this essay was able to optimize analysis of 3 SNP's. Due to the...
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Forensic SNP identification of trichological material by High Resolution Melting analysis
Strobachová, Joanne ; Stenzl, Vlastimil (advisor) ; Korabečná, Marie (referee)
The analysis of mitochondrial DNA (mtDNA) from shed hairs has gained high importance in forensic casework since telogen hairs are one of the common type of evidence left at the crime scene. This type of evidence very often lack of nuclear DNA (nDNA), so the analysis of mtDNA is the only possibility. The analysis of trichological material lacking nDNA is not commonly done in the forensic practice because of the high price of the regularly used mtDNA analysis method, as well as the time demandingness. For these reasons we decided to analyze the mtDNA and identify the trichological material by analysis of chosen single based polymorphism's (SNP) followed by High Resolution Melting (HRM). In order to verify the robustness of the HRM analysis method on several types of biological material that belongs to one person, we tested trichological material in anagen and telogen. From total number of 5 selected SNP's, this paper was able to optimize analysis of 3 SNP's. Due to the analysis of SNP C14766T (applicable for the trichological material in anagen and telogen), SNPs C7028T and G3010A (applicable only for the trichological material in anagen). The mtDNA sequences of different biological materials did not show intra-individual differences. Protocol that could be used for the forensic practice with the use...
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