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Interaction between NKT and myeloid derived suppressor cells and antitumour immunity
Straňavová, Lucia ; Reiniš, Milan (advisor) ; Holáň, Vladimír (referee)
Myeloid- derived suppressor cells (MDSCs) are a heterogeneous population of cells, which plays an important role in the suppression of anti-tumor immune responses. NKT cells represent an additional heterogeneous cell population that plays a crucial role in the regulation of immune responses. It shows that MDSCs and NKT cells may be similar to other populations imunoregulatory cells interact with each other and influence their functions. These interactions are important regulatory factor that may contribute to activation and to suppress anti-tumor immunity. Through interactions with type I NKT cells could differentiate these immunosuppressive MDSCs to immunogenic APC, which could form the basis for immunotherapeutic vaccine. All interactions between the NKT and MDSCs but have a positive effect of imunoregulatory. Interaction between MDSCs and CD4 + NKT cells II. type are immunosuppressive and may subsequently suppress the activity of cytotoxic T-lymphocyte (CTL). In some tumor models it was found that the immunosuppressive nature may also be interactions between MDSCs and type I NKT cells He had, however, alleviate the use of all-trans-retinol acid (ATRA), which induces differentiation of MDSCs.
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The role of memory T cells in transplant immunity
Straňavová, Lucia
Memory T cells represent a specific subpopulation of cells formed during the first encounter with antigen. The main role of these cells is to elicit faster and more effective secondary response during reinfections. In transplant immunity, they may affect graft survival directly with donor-specific memory T cells or with cross-reactive virus-specific memory T cells. In this study, we focused on donor-specific and CMV-specific memory/effector T cells. We were interested in the effect of immunosuppressive therapy on the frequency of these cells in periphery. We found that the immunosuppression, prophylaxis and length of dialysis did not significantly affect the number of CMV-reactive cells 6 months after transplantation. We were also interested in the cross-reactivity between CMV and donor antigens, so-called heterologous immunity, which we verified by analyzing the TCR-β repertoire using next- generation sequencing (NGS) in CMV and donor-reactive T cells. Functional cross-reactive T cell clones (shared the same TCR-β sequence) were then found both in the peripheral blood of pre-transplant patients and in the post-transplant graft biopsy. We were also interested if long-term dialysis treatment affects immune memory. Dialysis therapy is often associated with the presence of poorly defined immune system...
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The role of memory T cells in transplant immunity
Straňavová, Lucia ; Viklický, Ondřej (advisor) ; Zajícová, Alena (referee) ; Vlas, Tomáš (referee)
Memory T cells represent a specific subpopulation of cells formed during the first encounter with antigen. The main role of these cells is to elicit faster and more effective secondary response during reinfections. In transplant immunity, they may affect graft survival directly with donor-specific memory T cells or with cross-reactive virus-specific memory T cells. In this study, we focused on donor-specific and CMV-specific memory/effector T cells. We were interested in the effect of immunosuppressive therapy on the frequency of these cells in periphery. We found that the immunosuppression, prophylaxis and length of dialysis did not significantly affect the number of CMV-reactive cells 6 months after transplantation. We were also interested in the cross-reactivity between CMV and donor antigens, so-called heterologous immunity, which we verified by analyzing the TCR-β repertoire using next- generation sequencing (NGS) in CMV and donor-reactive T cells. Functional cross-reactive T cell clones (shared the same TCR-β sequence) were then found both in the peripheral blood of pre-transplant patients and in the post-transplant graft biopsy. We were also interested if long-term dialysis treatment affects immune memory. Dialysis therapy is often associated with the presence of poorly defined immune system...
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Adhesion, growth and potential immune activation of cells on metallic materials for bone implants.
Straňavová, Lucia ; Bačáková, Lucie (advisor) ; Filová, Elena (referee)
The contemporary orthopaedics and traumatology of the musculoskeletal system and stomatology have been witnessing a substantial increase in the number of surgeries using metallic implants. The issue of reconstruction of bone defects covers a large area of study, where the surface properties of the implants are extremely important. Bone defects often occur as a result of open fractures, radical cancer treatment or limb lengthening, which is very common in paediatric orthopaedics. In the treatment of these conditions, the surface of the applied materials should provide a favorable environment for bone cells and support bone formation. In endoprosthetics it is highly desirable to achieve the strongest possible fixation between the implant surface and the bone. During the surgery, primary stability of the implant fixation is ensured by the proper positioning of the implant, based on the appropriate shape of the implant and the quality of bone cut. The initial stability is only temporary, being estimated to last approximately three months. After this period, the secondary stability starts, determined by the bone ingrowth into the implant surface structure. Osteogenic differentiation and extracellular matrix (ECM) mineralization can be enhanced by the presence of bone morphogenetic proteins (BMPs),...
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Interaction between NKT and myeloid derived suppressor cells and antitumour immunity
Straňavová, Lucia ; Reiniš, Milan (advisor) ; Holáň, Vladimír (referee)
Myeloid- derived suppressor cells (MDSCs) are a heterogeneous population of cells, which plays an important role in the suppression of anti-tumor immune responses. NKT cells represent an additional heterogeneous cell population that plays a crucial role in the regulation of immune responses. It shows that MDSCs and NKT cells may be similar to other populations imunoregulatory cells interact with each other and influence their functions. These interactions are important regulatory factor that may contribute to activation and to suppress anti-tumor immunity. Through interactions with type I NKT cells could differentiate these immunosuppressive MDSCs to immunogenic APC, which could form the basis for immunotherapeutic vaccine. All interactions between the NKT and MDSCs but have a positive effect of imunoregulatory. Interaction between MDSCs and CD4 + NKT cells II. type are immunosuppressive and may subsequently suppress the activity of cytotoxic T-lymphocyte (CTL). In some tumor models it was found that the immunosuppressive nature may also be interactions between MDSCs and type I NKT cells He had, however, alleviate the use of all-trans-retinol acid (ATRA), which induces differentiation of MDSCs.
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