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Study of barrier lipids in the skin and skin models
Sochorová, Michaela ; Vávrová, Kateřina (advisor) ; Zimčík, Petr (referee) ; Salavec, Miloslav (referee)
1 Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Candidate: Mgr. Michaela Sochorová Supervisor: prof. PharmDr. Kateřina Vávrová, Ph.D. Title of doctoral thesis: Study of barrier lipids in the skin and skin models The barrier lipids are found in the intercellular spaces of the uppermost layer of epidermis - stratum corneum (SC). These lipids comprise an approximately equimolar mixture of ceramides (Cer), fatty acids (FA) and cholesterol (Chol). The composition and organization of the lipid mixture is unique and provides a barrier, which protects organism against harmful effects from the surroundings and, at the same time, it protects body from excessive water loss and contributes to homeostasis maintenance. Skin barrier disruption, including altered barrier lipid composition and organization, was described in many skin diseases, for example atopic dermatitis, psoriasis or skin cancer. Study and therapy of these diseases are limited by their complex pathophysiology and a lack of biological material (human skin). Therefore, skin models are appropriate and useful tools for study of skin diseases. The skin models differ in their simplicity/complexity and their preparation therefore they can provide different information. Model lipid membranes are...
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Shamanism from the View of the Theories of Religious Studies
Sochorová, Michaela ; Gebelt, Jiří (advisor) ; Obuchová, Ľubica (referee)
This work is concerned with the origin, development and problems connected with the concept of shamanism. The originally indigenous term was transformed from its objective level to the level of scientific metalanguage and soon after that became a universal concept, which according to the postmodern critics of too generalized and unclear terms was rejected as empty and meaningless. It is the consequence of universal definitions that not only made "shamanism" popular, but also inspired the interest for this phenomenon among many different disciplines, which always reflect "shamanism" in a number of various ways. The phenomenon of neoshamanism and the revitalization of the native "shamanic" traditions also have to be taken into consideration. Thanks to that it is really hard to define the meaning of the term itself. The aim of this work is at first to introduce - on the basis of a typology - the main, i.e. most widespread approaches working with the concept of shamanism, defining it and at the same time providing some propositions concerning the solution of the problems mentioned above. I also try to answer the question wheather the "shamanism" still represents a contributive category for contemporary science.
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Study of barrier lipids in the skin and skin models
Sochorová, Michaela ; Vávrová, Kateřina (advisor) ; Zimčík, Petr (referee) ; Salavec, Miloslav (referee)
1 Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Candidate: Mgr. Michaela Sochorová Supervisor: prof. PharmDr. Kateřina Vávrová, Ph.D. Title of doctoral thesis: Study of barrier lipids in the skin and skin models The barrier lipids are found in the intercellular spaces of the uppermost layer of epidermis - stratum corneum (SC). These lipids comprise an approximately equimolar mixture of ceramides (Cer), fatty acids (FA) and cholesterol (Chol). The composition and organization of the lipid mixture is unique and provides a barrier, which protects organism against harmful effects from the surroundings and, at the same time, it protects body from excessive water loss and contributes to homeostasis maintenance. Skin barrier disruption, including altered barrier lipid composition and organization, was described in many skin diseases, for example atopic dermatitis, psoriasis or skin cancer. Study and therapy of these diseases are limited by their complex pathophysiology and a lack of biological material (human skin). Therefore, skin models are appropriate and useful tools for study of skin diseases. The skin models differ in their simplicity/complexity and their preparation therefore they can provide different information. Model lipid membranes are...
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Study of barrier lipids in the skin and skin models
Sochorová, Michaela ; Vávrová, Kateřina (advisor) ; Zimčík, Petr (referee) ; Salavec, Miloslav (referee)
1 Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Candidate: Mgr. Michaela Sochorová Supervisor: prof. PharmDr. Kateřina Vávrová, Ph.D. Title of doctoral thesis: Study of barrier lipids in the skin and skin models The barrier lipids are found in the intercellular spaces of the uppermost layer of epidermis - stratum corneum (SC). These lipids comprise an approximately equimolar mixture of ceramides (Cer), fatty acids (FA) and cholesterol (Chol). The composition and organization of the lipid mixture is unique and provides a barrier, which protects organism against harmful effects from the surroundings and, at the same time, it protects body from excessive water loss and contributes to homeostasis maintenance. Skin barrier disruption, including altered barrier lipid composition and organization, was described in many skin diseases, for example atopic dermatitis, psoriasis or skin cancer. Study and therapy of these diseases are limited by their complex pathophysiology and a lack of biological material (human skin). Therefore, skin models are appropriate and useful tools for study of skin diseases. The skin models differ in their simplicity/complexity and their preparation therefore they can provide different information. Model lipid membranes are...
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EEG ERP Response Inhibition of Selective Attention in Bilinguals
SOCHOROVÁ, Michaela
This Bachelor's Degree thesis studies the difference in basic processing processes between bilinguals and monolinguals. Specifically, it examines executive functions and selective attention. In the theoretical portion, elaborated information including speech development, bilingualism, electroencephalography and even-related potentials is explored. The practical portion of the study explores the metodology and analysis of data obtained from the experiment. The function and attention experiment presents test subjects with a letter-detection task during which simultaneous tones are presented at 76 dB sound pressure level. The experiment uses two groups of test subjects. An experimental group of Czech-English bilinguals and a control group of Czech monolinguals. Both groups underwent the same experimental design. Two types of data, behavioral and EEG data in the form of even-related potentials, were collected from the experiment. Behavioral data was processed in the STATISTICA program using neparametric tests. EEG data was analyzed in the MATLAB program. Results from statistical analysis of the behavioral data did not show a difference between the subject groups in the number of errors or length of response time. Statistical analysis of the EEG data showed a significant difference between the groups; however overall, the study resulted in a difficult determination and an unclear interpretation of the results.
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Analysis of lipids in epidermis with filaggrin knock-down
Sochorová, Michaela ; Vávrová, Kateřina (advisor) ; Hrabálek, Alexandr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Inorganic and Organic Chemistry Michaela Sochorová Supervisor: Doc. PharmDr. Kateřina Vávrová, Ph.D. Title of Diploma thesis: Analysis of lipids in epidermis with filaggrin knock-down Filaggrin (FLG) is crucial for correct development of epidermis and for its function as a skin barrier. Mutation in the FLG gene and loss of its function is associated with diseases such as ichthyosis vulgaris and atopic dermatitis. The aim of the study was to evaluate the impact of FLG knock-down on the composition of intercellular lipids in the stratum corneum (SC). We used in vitro skin constructs with reduced (FLG-) and normal gene expression (FLG+) for the analysis. It enabled us to study separately the influence of FLG on the composition and organization of SC lipids. FLG is a protein which is degraded to metabolites which cause an acidification of SC. pH is important for optimal activity of enzymes which are pivotal for formation of ceramides from their precursors. Therefore we assumed lower ceramide content in skin barrier when FLG is reduced. Our assumption was supported with findings in patients with atopic dermatitis. However our HPTLC analysis showed that the amount of lipids is similar in both FLG- and FLG+ skin construct....
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Shamanism from the View of the Theories of Religious Studies
Sochorová, Michaela ; Gebelt, Jiří (advisor) ; Obuchová, Ľubica (referee)
This work is concerned with the origin, development and problems connected with the concept of shamanism. The originally indigenous term was transformed from its objective level to the level of scientific metalanguage and soon after that became a universal concept, which according to the postmodern critics of too generalized and unclear terms was rejected as empty and meaningless. It is the consequence of universal definitions that not only made "shamanism" popular, but also inspired the interest for this phenomenon among many different disciplines, which always reflect "shamanism" in a number of various ways. The phenomenon of neoshamanism and the revitalization of the native "shamanic" traditions also have to be taken into consideration. Thanks to that it is really hard to define the meaning of the term itself. The aim of this work is at first to introduce - on the basis of a typology - the main, i.e. most widespread approaches working with the concept of shamanism, defining it and at the same time providing some propositions concerning the solution of the problems mentioned above. I also try to answer the question wheather the "shamanism" still represents a contributive category for contemporary science.
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