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Study of mesenchymal stem cell migration in the extracellular matrix based on principles of chemotaxis
Scholasterová, Viktorie ; Fohlerová, Zdenka (referee) ; Chmelíková, Larisa (advisor)
This thesis engages in a study of mesenchymal stem cell migration in extracellular matrix based on principles of chemotaxis. First, attention is focused on a theoretical part associated with a clarification of basic terms such as extracellular matrix, migration, confocal microscopy, mesenchymal stem cells or chemotaxis. There is also included a list and a description of some basic methods for monitoring cell migration and a more detailed description of a method called transwell assay, which has been chosen for an experiment in a practical part of this thesis. This part includes protocols of individual steps for the preparation of the experiment, the procedure of data processing obtained by scanning cells with a confocal microscope and a description of the resulting confluence values.
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ECG quality evaluation
Scholasterová, Viktorie ; Němcová, Andrea (referee) ; Smital, Lukáš (advisor)
This bachelor’s thesis deals with ECG quality evaluation. Its main goal is to examine the theoretical basis for a realization of selected methods for ECG quality evaluation. In the theoretical part of the thesis there is described electrocardiography, morphology of electrocardiogram, types of signal interference and Holter recorders. A basic procedure of ECG quality evaluation including filtration, detection of important characteristics of ECG signal and an overview of chosen methods follows. The practical part contains a description of three methods for ECG quality evaluation – a method of average heart rate, a method of calculation signal-to-noise ratio (SNR) and a method of band-pass filters. Finally, the results of the methods using artificial data, real-time data and data from a competition PhysioNet/Computing in Cardiology Challenge 2011 are evaluated.
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Study of mesenchymal stem cell migration in the extracellular matrix based on principles of chemotaxis
Scholasterová, Viktorie ; Fohlerová, Zdenka (referee) ; Chmelíková, Larisa (advisor)
This thesis engages in a study of mesenchymal stem cell migration in extracellular matrix based on principles of chemotaxis. First, attention is focused on a theoretical part associated with a clarification of basic terms such as extracellular matrix, migration, confocal microscopy, mesenchymal stem cells or chemotaxis. There is also included a list and a description of some basic methods for monitoring cell migration and a more detailed description of a method called transwell assay, which has been chosen for an experiment in a practical part of this thesis. This part includes protocols of individual steps for the preparation of the experiment, the procedure of data processing obtained by scanning cells with a confocal microscope and a description of the resulting confluence values.
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ECG quality evaluation
Scholasterová, Viktorie ; Němcová, Andrea (referee) ; Smital, Lukáš (advisor)
This bachelor’s thesis deals with ECG quality evaluation. Its main goal is to examine the theoretical basis for a realization of selected methods for ECG quality evaluation. In the theoretical part of the thesis there is described electrocardiography, morphology of electrocardiogram, types of signal interference and Holter recorders. A basic procedure of ECG quality evaluation including filtration, detection of important characteristics of ECG signal and an overview of chosen methods follows. The practical part contains a description of three methods for ECG quality evaluation – a method of average heart rate, a method of calculation signal-to-noise ratio (SNR) and a method of band-pass filters. Finally, the results of the methods using artificial data, real-time data and data from a competition PhysioNet/Computing in Cardiology Challenge 2011 are evaluated.
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