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Spectral Analysis in Microscopy
Sýs, Michal ; Procházková, Jana (referee) ; Štarha, Pavel (advisor)
The aim of this work is to develop a mathematical apparatus for the accurate description of the colour of objects in a microscope image. This problem is solved by extracting the brightness intensities of pollen grains at fourteen wavelength bands and representing them in color space. The developed apparatus has been successfully applied to two series of images, allowing comparison of the color of individual objects and simplifying its description. The results obtained allow accurate investigation of the colour of objects in the image and form a simple tool for spectral analysis.
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UV-Vis and IR Spectroelectrocbemistry of Copper Complexes and Bioactive Compounds
Sokolová, Romana ; Obluková, Michaela ; Sýs, M. ; Mikysek, T. ; Wantulok, J. ; Nycz, J. E. ; Degano, I.
The interpretation of the change of absorption spectrum of an oxidized and reduced molecule\nrecorded during the electron transfer is an efficient tool for the determination of oxidation or\nreduction mechanism. This technique provides the information about the electroactive\nchromophore and is performed in two regimes of electrochemical measurement, cyclic\nvoltammetry and chronoamperometry, respectively. This approach was successfully applied to\nstudy the fundamental electrochemical behavior of recently synthesized copper complexes\nproviding biomimetic activity, ofpolyphenolic bioactive compounds, and also for the reduction\nof halogenated phenanthrolines. The identification of redox products was done by\nchromatographic techniques as HPLC-DAD and HPLC-ESI-MS/MS.
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Spectral Analysis in Microscopy
Sýs, Michal ; Procházková, Jana (referee) ; Štarha, Pavel (advisor)
The aim of this work is to develop a mathematical apparatus for the accurate description of the colour of objects in a microscope image. This problem is solved by extracting the brightness intensities of pollen grains at fourteen wavelength bands and representing them in color space. The developed apparatus has been successfully applied to two series of images, allowing comparison of the color of individual objects and simplifying its description. The results obtained allow accurate investigation of the colour of objects in the image and form a simple tool for spectral analysis.
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