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The use of GC/MS for the analysis of drugs
Sýkora, Richard ; Opatřilová, Radka (referee) ; Vávrová, Milada (advisor)
This diploma thesis is based on the current issue of the presence of pharmaceuticals in various components of the environment. Concerning the contamination by residues of pharmaceuticals the most affected environment is the aquatic environment where these substances leaks especially from wastewater treatment plants, which eliminate them during the cleaning process only partially. This work is focused on the selected group of pharmaceuticals, non-steroidal anti-inflammatory drugs (salicylic acid, ibuprofen, caffeine, naproxen, ketoprofen, diclofenac) in waste water. For analysis purposes two types of sampling were used and compared: the conventional spot sampling of wastewater and the sampling using passive samplers POCIS. The sampling took place at the inflow and outflow of the wastewater treatment plant in Brno Modřice. The solid phase extraction (SPE) using Oasis HLB columns was used as the extraction method. Extracted sample was derivatized then. Derivatization agents were: MSTFA (N-methyl-N-(trimethylsilyl)trifluoroacetamid) and BSTFA (N, O-bis(trimethylsilyl) trifluoroacetamid). The final analysis was performed using gas chromatography with mass spectrometric detection Time-of-Flight (GC/TOF-MS).
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Application of gas chromatography for determination of drug residuals
Sýkora, Richard ; Lisá, Hana (referee) ; Vávrová, Milada (advisor)
Analgesics are nowadays widely used group of drugs in human and also in veterinary medicine. Residuals of these drugs enter surface waters via discharges from the waste water treatment plants as the result of insufficient efficiency of their removal during cleaning process. In this bachelor thesis the possibilities of the analgesics residuals determination in waters using derivatization followed by gas chromatography with mass spectrometric detection were experimentally evaluated. Individual derivatization procedures were compared too. Several derivatization agents were tested and experimental parameters for selected analgesics were optimized (reaction time and temperature, amount of derivatizing agent). Further, several procedures of isolation of target compounds from water based on Solid Phase Extraction were verified. In all cases, gas chromatography with TOF-based mass spectrometric detection acts as final analytical method.
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The use of GC/MS for the analysis of drugs
Sýkora, Richard ; Opatřilová, Radka (referee) ; Vávrová, Milada (advisor)
This diploma thesis is based on the current issue of the presence of pharmaceuticals in various components of the environment. Concerning the contamination by residues of pharmaceuticals the most affected environment is the aquatic environment where these substances leaks especially from wastewater treatment plants, which eliminate them during the cleaning process only partially. This work is focused on the selected group of pharmaceuticals, non-steroidal anti-inflammatory drugs (salicylic acid, ibuprofen, caffeine, naproxen, ketoprofen, diclofenac) in waste water. For analysis purposes two types of sampling were used and compared: the conventional spot sampling of wastewater and the sampling using passive samplers POCIS. The sampling took place at the inflow and outflow of the wastewater treatment plant in Brno Modřice. The solid phase extraction (SPE) using Oasis HLB columns was used as the extraction method. Extracted sample was derivatized then. Derivatization agents were: MSTFA (N-methyl-N-(trimethylsilyl)trifluoroacetamid) and BSTFA (N, O-bis(trimethylsilyl) trifluoroacetamid). The final analysis was performed using gas chromatography with mass spectrometric detection Time-of-Flight (GC/TOF-MS).
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Application of gas chromatography for determination of drug residuals
Sýkora, Richard ; Lisá, Hana (referee) ; Vávrová, Milada (advisor)
Analgesics are nowadays widely used group of drugs in human and also in veterinary medicine. Residuals of these drugs enter surface waters via discharges from the waste water treatment plants as the result of insufficient efficiency of their removal during cleaning process. In this bachelor thesis the possibilities of the analgesics residuals determination in waters using derivatization followed by gas chromatography with mass spectrometric detection were experimentally evaluated. Individual derivatization procedures were compared too. Several derivatization agents were tested and experimental parameters for selected analgesics were optimized (reaction time and temperature, amount of derivatizing agent). Further, several procedures of isolation of target compounds from water based on Solid Phase Extraction were verified. In all cases, gas chromatography with TOF-based mass spectrometric detection acts as final analytical method.
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