|
|
Nanoparticles and Their Effect on the Immune System: Study of the Proinflammatory Potential of Selected Carbon Nanomaterials
Švadláková, Tereza ; Krejsek, Jan (advisor) ; Stulík, Jiří (referee) ; Roušar, Tomáš (referee)
Carbon-based nanomaterials (CNMs) have unique physical-chemical properties, which make them appropriate candidates for both industry and medicine. However along with production, there are growing concerns about their effects on human organism. For this reason, CNMs are frequent topic of toxicological studies. A key step in the clarification of their safety is to evaluate their interaction with the components of the immune system, particularly their ability to cause inflammation. For some allotropes e.g., pristine graphene derivatives, significant results are still missing or incomplete. For these reasons, this dissertation deals with the evaluation of the proinflammatory effect of two types of pristine graphene platelets (GPs), which represent common intermediate in the processing of other graphene derivatives, and which can penetrate to body via inhalation. For comparison, our work also includes an evaluation of the proinflammatory effect of multiwalled carbon nanotubes (MWCNTs). Professional phagocytes, particularly monocytes and macrophages represent key cells in processing and elimination of foreign and damaged or abnormal elements. Since phagocytes also represent main mediators of inflammation, we selected human primary monocytes and human monocytic cell line THP-1 differentiated in macrophages...
|
|
|
Nephrotoxicity testing in vitro
Handl, Jiří ; Roušar, Tomáš (advisor) ; Patočka, Jiří (referee) ; Andrýs, Rudolf (referee)
2020 UNIVERSITY OF PARDUBICE FACULTY OF CHEMICAL TECHNOLOGY Doctoral Thesis: NEPHROTOXICITY TESTING IN VITRO Author: Mgr. Jiří Handl, Ph.D. Supervisor: doc. RNDr. Tomáš Roušar, Ph.D. Abstract The doctoral thesis deals with in vitro testing of nephrotoxic effects of substances in immortalized human proximal tubular cells HK-2. The basic aim of the thesis was to introduce and optimize the cultivation of HK-2 cells under different culture conditions, to characterize the cultivation and growth stability of HK-2 cells and last but not least to characterize the nephrotoxic effect of cadmium in HK-2 cell line using bioanalytical and biochemical methods. The introduction summarizes the basic rules and conditions for long-term cultivation of cell lines. Furthermore, HK-2 cells and their importance in nephrotoxicity testing are characterized in detail. The thesis also mentions methods for monitoring toxic effects of substances with focus on measuring cell viability and oxidative stress. The outcomes of the thesis are characterizing nephrotoxic effects of CdCl2 in HK-2 cells. The results part describes obtained findings and the discussions follows. Keywords Nephrotoxicity; In vitro cultivation; HK-2 cell line; Cell viability; Cadmium.
|
|
| |
|
| |
|
| |
guest ::
