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Determination of Total Antioxidant Capacity in Select Food products and Dietary Supplements by Flow Injection Analysis with Chemiluminiscent Detection
Průchová, Karolína ; Rychlovský, Petr (advisor) ; Hraníček, Jakub (referee)
In this diploma thesis, a functional method for the determination of total antioxidant capacity by flow injection analysis with the chemiluminiscent detection was successfully developed. Luminol in carbonate buffer (composed of Na2CO3, NaHCO3 and (NH4)2CO3), with Cu2+ ionts as a catalyst, was used as a chemiluminiscent reagent. Chemiluminiscent radiation was induced by a diluted solution of hydrogen peroxide, a long-lasting chemiluminiscence was observed. First part of this thesis is dedicated to the optimalisation of the experimantal conditions for antioxidant capacity measurements as well as to the construction of apparatus for flow measurings. L-ascorbic acid was used as a standard. In the second part of this thesis the freshly developed method was used to determinate total antioxidant capacity of real food product simples, namely tea and coffee simples, beers, wines, chocolates, fresh fruit juices and selected food supplements. Results of this analysis were expressed as a vitamin C equivalent antioxidant capacity (VCEAC) related to standard, in mg/ml for liquid simples and in g/mg for solid simples. Results obtained are: tea simples 53 - 347 mg/1g of dried product, coffee simples 399-449 mg/g of dried product, beers 1,1-1,4 mg/ml, wines 4,2 - 4,8 mg/ml, fresh lemon juice 4,7 mg/ml, fresh kiwi juice 2,1...
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