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Utilization of tissue cultures for toxicology of the environment.
Polanská, Daniela ; Cajthaml, Tomáš (advisor) ; Klusoň, Petr (referee)
5 Abstract Five substances from the group of so-called personal care products, known for their low degradability and regular environmental detection, were tested for toxicity using two fish tissue lines (RTgill-W1 a RTG-2) isolated from rainbow trout (Oncorhynchus miykiss). The tested substances were hexadecylpyridinium chloride (HDP), chlorhexidine (CHX), octenidine (OCT), thymol (THM) and triclosan (TCS). A cell viability assay was performed with each of these compounds using Alamar Blue ™ (AB), 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM) and neutral red (NR) protocols. The results were used to construct dose-response curves along with an EC50 value for each of these substances. The EC50 values ranged from 0,51 (HDP) to 33,75 µg.ml-1 (THM) for RTgill-W1 and from 0,31 (HDP) to 33,37 µg.ml-1 (THM) for RTG-2. The theoretical LC50 estimation was calculated according to Tanneberger et al. (2013). For all substances, cytochrome P450 1A activity was monitored using 7-ethoxyresorufin-o-deethylase (EROD), four out of five tested chemicals were statistically positive for EROD, the highest EROD response was observed for the most toxic compound - HDP. Only TCS did not show statistically significant cytochrome P450 1A activity. In addition, oxidative stress was measured with the fluorescent dye...
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Basic haematological parameters and their influencing by pollutants in common carp a review
POLANSKÁ, Daniela
The objective of this study was to describe the methods of determining the main haematological parameters and obtain as much information as possible about pollutants that may influence them. The subject of this thesis is the common carp (Cyprinus carpio L.). As far as pollutants are concerned, I focused on nitrogen compounds (ammonia, nitrite and cyanide), metals (Zn, Cu, Fe), heavy metals (Hg, Cd, Pb, Cr), petroleum substances, pesticides (organophosphates, triazines and diazines, pyrethroids), pharmaceuticals and natural supplements and persistent organic pollutants. Collected information imply that heavy metals and pesticides have the most important effect on haematological parameters of the common carp. Heavy metals cause changes in haematological parameters and cell anomalies and pesticides may cause haematopoietic failure, which especially means a decrease of number of red blood cells, the concentration of haemoglobin, the haematocrit value and the changes in the presence of different leukocyte forms. This study summarizes the knowledge of the effects of the aforementioned pollutants and highlights the danger of their effect as well as the possible threat to fish health, not just of the common carp. The information dealing with the effects of many pollutants on the blood parameters of fish is missing in available scientific literature.
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