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Determination of selected phenolic compounds in fruit
Pechová, Martina ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department of Analytical Chemistry Candidate: Bc. Martina Pechová Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Determination of selected phenolic compounds in fruit This diploma work deals with development and optimization of HPLC method for determination of selected phenolic compounds - gallic acid, chlorogenic acid, caffeic acid, catechin, epicatechin, rutin, quercetin, quercitrin, phloretin and phloridzin. The diploma work includes information about the general characteristics of phenolic compounds and information about their structure. It further describes HPLC method and mentions the short review of scientific publications regarding this topic. In the optimization step several types of gradients of mobile phase, stationary phase (chromatographic column of type C18, phenyl-hexyl, biphenyl, amino, cyano and monolithic column), other separation conditions (temperature, extraction) were tested and validation of the method was carried out. For the analysis of selected compounds precolumn Ascentis Express C18 (5 x 4,6 mm x 5 µm) and the column Kinetex C18 column (150 x 4,6 mm x 5 µm) were used. The detection was performed by DAD spectrophotometric detector at wavelengths of 255, 280, 320 and 365 nm, column...
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Determination of selected phenolic compounds in fruit
Pechová, Martina ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department of Analytical Chemistry Candidate: Bc. Martina Pechová Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Determination of selected phenolic compounds in fruit This diploma work deals with development and optimization of HPLC method for determination of selected phenolic compounds - gallic acid, chlorogenic acid, caffeic acid, catechin, epicatechin, rutin, quercetin, quercitrin, phloretin and phloridzin. The diploma work includes information about the general characteristics of phenolic compounds and information about their structure. It further describes HPLC method and mentions the short review of scientific publications regarding this topic. In the optimization step several types of gradients of mobile phase, stationary phase (chromatographic column of type C18, phenyl-hexyl, biphenyl, amino, cyano and monolithic column), other separation conditions (temperature, extraction) were tested and validation of the method was carried out. For the analysis of selected compounds precolumn Ascentis Express C18 (5 x 4,6 mm x 5 µm) and the column Kinetex C18 column (150 x 4,6 mm x 5 µm) were used. The detection was performed by DAD spectrophotometric detector at wavelengths of 255, 280, 320 and 365 nm, column...
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Determination of selected phenolic compounds in fruit
Pechová, Martina ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department of Analytical Chemistry Candidate: Bc. Martina Pechová Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Determination of selected phenolic compounds in fruit This diploma work deals with development and optimization of HPLC method for determination of selected phenolic compounds - gallic acid, chlorogenic acid, caffeic acid, catechin, epicatechin, rutin, quercetin, quercitrin, phloretin and phloridzin. The diploma work includes information about the general characteristics of phenolic compounds and information about their structure. It further describes HPLC method and mentions the short review of scientific publications regarding this topic. In the optimization step several types of gradients of mobile phase, stationary phase (chromatographic column of type C18, phenyl-hexyl, biphenyl, amino, cyano and monolithic column), other separation conditions (temperature, extraction) were tested and validation of the method was carried out. For the analysis of selected compounds precolumn Ascentis Express C18 (5 x 4,6 mm x 5 µm) and the column Kinetex C18 column (150 x 4,6 mm x 5 µm) were used. The detection was performed by DAD spectrophotometric detector at wavelengths of 255, 280, 320 and 365 nm, column...
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Musicotherapy from the perspective of learning and behavioral disorders
PECHOVÁ, Martina
The goal of my diploma thesis is introduce one of alternative direction {--} music therapy, to outline the sphere of specific learning and behavior disorders, and also to give a guide, how to apply components of music therapy during the work with children with these disorders. The thesis consists of two parts {--} a theoretical and practical part. The theoretical part focuses on defining key terms, such as music therapy, dyslexia, dysgraphia, dysortographia, dyscalculia, dyspinxia, dysmusia, dyspraxia. In my work I aim to clarify the question of ADHD and try to point out the relation of particular learning and behavior disorders. The practical part includes twenty activities that were made firstly according to a methodical manual written by M. Beníčková and Z. Vilímek, who use the Czech method of music therapy {--} Music of body, and secondly on the base of casuistry of two boys attending special practical school. Each activitiy should develop particular skills, comprises tools, motivation, its own description and analysis coming out of a practical accomplishment. The method of observing was used for the evaluation.
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