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Diagnostic protocol for Erwinia amylovora, the causal agent of fire blight
Kokošková, B. ; Mráz, Ivan
The aim of this methodology is optimized diagnostic protocol for detection and determination of the causal agent of fire blight, quarantine bacterium E. amylovora. The disease causes high economic losses in plantations of pome-fruit trees and susceptible ornamental plants, in particular in Crataegus sp. and Cotoneaster sp. Diagnostic protocol is based on a combination of both optimized techniques utilizing different principles of action: immunochemical (immunofluorescent test) and molecular (polymerase chain reaction). The submitted diagnostic protocol alows to eliminate potential false negatives and positives and ensure the highest reliability of results. Specific and timely detection of E. amylovora alows effective application of plant protection measures.
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Diagnostic method for Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial wilt of tomato
Kokošková, B. ; Mráz, Ivan ; Fousek, Jan
The aim of this methodology was optimize detection and determination of quarantine bacterium Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato, The disease causes high ecomomic losses in main production areas on indeterminate greenhouse and fi eld tomatoes. Methodology is based on a combination of both optimized techniques utilizing different principles of action: immunochemical (IF) and molecular (PCR). The submitted diagnostic protocol alows to eliminate potential false negatives and positives and ensure the highest reliability of results. Specific and timely detection of Clavibacter michiganensis subsp. michiganensis will alow effective aplication of plant protection mesures.
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