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Sequential injection chromatography-testing and comparison of modern chromatographic columns
Molnárová, Petra ; Chocholouš, Petr (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Deparment of Analytical chemismy Candidate: Bc. Petra Molnárová Supervisor: PharmDr. Petr Chocholouš, PhD. Title of diploma thesis: Sequential injection chromatogramy - testing and comparison of modern chromatographic columns This diploma thesis deals with testing and comparison of two modern chromatoghraphic columns in Sequential injection chromatography system. They were used for separation of selected vitamins B (thiamine, nikotinamide, pyridoxine, riboflavin and folic acid) and vitamin C. Developed method was based on UV detection at 220, 272 and 290nm. The Ascentis Express HILIC column (3 cm x 4.6 mm, 2.7 µm) was tested by using acetonitrile with ammonium acetate as a mobile phase. The method was not siccessfully developed because of low retention times of vitamins. The separation of six vitamins was not able, because of low selectivity. There was developed a method with use of Ascentis Express RP- Amide column (3 cm x 4.6 mm, 2.7 µm) using gradient elution, which was able to separate all 6 vitamins. Acetonitrile and phosphoric acid (pH 1.8) were components of the mobile phases. There was made calibration, reproducibility and recovery tests, but there was not good results enough to validate the method. For recovery was used...
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Antimicrobial effect of quaternary ammonium compounds
Molnárová, Petra ; Jílek, Petr (advisor) ; Vejsová, Marcela (referee)
Petra Molnárová Antimicrobial effect of quaternary ammonium compounds Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Healthcare bioanalytics/Medical Laboratory Technician This bachelor thesis deals with the detection of antimicrobial effect of quaternary ammonium compounds. For objective comparison and evaluation were the results compared with the effect of disinfectant which is not containing quaternary ammonium compounds. The entire experiment was conducted in microtitrative plates by micromethod based on Kneifl (1988). Some parts of the method have been optimalised according to our conditions and needs. We expressed the efficiency with values of minimal mikrobicidal concentration. Every experiment has been conducted several times because of the need to eliminate eventual errors as far as possible. In the results were compiled the average values. The most effective was disinfectant with the combined composition, which contains both quaternary ammonium compounds and another effective substances (aldehyde, acides,...), increasing it's effect. The other substances with quaternary ammonium compounds have been severalfold effective than comparative disinfectants. Very low levels of MBC indicated very clearly, that the disinfectans based on quaternary ammonium salts...
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Sequential injection chromatography-testing and comparison of modern chromatographic columns
Molnárová, Petra ; Chocholouš, Petr (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Deparment of Analytical chemismy Candidate: Bc. Petra Molnárová Supervisor: PharmDr. Petr Chocholouš, PhD. Title of diploma thesis: Sequential injection chromatogramy - testing and comparison of modern chromatographic columns This diploma thesis deals with testing and comparison of two modern chromatoghraphic columns in Sequential injection chromatography system. They were used for separation of selected vitamins B (thiamine, nikotinamide, pyridoxine, riboflavin and folic acid) and vitamin C. Developed method was based on UV detection at 220, 272 and 290nm. The Ascentis Express HILIC column (3 cm x 4.6 mm, 2.7 µm) was tested by using acetonitrile with ammonium acetate as a mobile phase. The method was not siccessfully developed because of low retention times of vitamins. The separation of six vitamins was not able, because of low selectivity. There was developed a method with use of Ascentis Express RP- Amide column (3 cm x 4.6 mm, 2.7 µm) using gradient elution, which was able to separate all 6 vitamins. Acetonitrile and phosphoric acid (pH 1.8) were components of the mobile phases. There was made calibration, reproducibility and recovery tests, but there was not good results enough to validate the method. For recovery was used...
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Antimicrobial effect of quaternary ammonium compounds
Molnárová, Petra ; Jílek, Petr (advisor) ; Vejsová, Marcela (referee)
Petra Molnárová Antimicrobial effect of quaternary ammonium compounds Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Healthcare bioanalytics/Medical Laboratory Technician This bachelor thesis deals with the detection of antimicrobial effect of quaternary ammonium compounds. For objective comparison and evaluation were the results compared with the effect of disinfectant which is not containing quaternary ammonium compounds. The entire experiment was conducted in microtitrative plates by micromethod based on Kneifl (1988). Some parts of the method have been optimalised according to our conditions and needs. We expressed the efficiency with values of minimal mikrobicidal concentration. Every experiment has been conducted several times because of the need to eliminate eventual errors as far as possible. In the results were compiled the average values. The most effective was disinfectant with the combined composition, which contains both quaternary ammonium compounds and another effective substances (aldehyde, acides,...), increasing it's effect. The other substances with quaternary ammonium compounds have been severalfold effective than comparative disinfectants. Very low levels of MBC indicated very clearly, that the disinfectans based on quaternary ammonium salts...
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