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Analytical evaluation of drugs using HPLC IV.
Mojšová, Kristína ; Mokrý, Milan (advisor) ; Nobilis, Milan (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Candidate: Kristína Mojšová Supervisor: RNDr. Milan Mokrý, CSc. Title of master's thesis: Analytical evaluation of drugs using HPLC IV. This thesis describes development of the suitable method for ketoprofen analysis by high performance liquid chromatography. For this aim a LiChro CART® 125-4 Lichrospher® 100 RP-18 (5 μm) column was used as a stationary phase and mobile phase contained mixture of methanol and aqueous KH2PO4 buffer (pH 3,4) in a ratio of 60:40 (v/v). The flow rate was set to 1 ml/min, temperature on the column was 25 řC and substances were detected at a wave length of 254 nm. Under these conditions final retention time of ketoprofen was 7,9 minutes. Benetazon was chosen as an appropriate internal standard for the quantitative evaluation. In the next part we were looking for suitable conditions for the chiral separation of the analyzed drug by using normal and reverse mode. Separation of ketoprofen's enantiomers was carried out on a Chiralcel OD-R, 250 x 4,6 mm, from Daicel Chemical Industries using a mobile phase of heptane and 2-propanol in a ratio of 97:3, with the addition of 0,6 ml formic acid. At a flow rate of 0,7 ml/min, retention times took about 20 minutes....
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