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Mechanisms and regulation of callose deposition to the cell wall of Arabidopsis thaliana
Modráčková, Jana ; Kulich, Ivan (advisor) ; Šustr, Marek (referee)
Callose (β-1,3-glucan) is a significant plant cell wall polysaccharide, which participates in many developmental and stress responses. Despite the importance of this polymer, the knowledge on the causes of callose synthesis, its regulation and the meaning of callose deposition are still limited. The family of callose synthases was revealed thanks to molecular and genetic methods. They are responsible for callose deposition in diferent reactions and the diferent spaces of the plant body. This thesis summarizes the knowleadge about the processes, in which callose is involved, and it presents an overview of the individual proteins from callose synthase family including their phylogenetic analysis and the comparison with the cellulose synthase focusing on the model plant Arabidopsis thaliana. Key words: Arabidopsis thaliana, β-1,3-glucan, callose, callose synthase, plant cell wall polymer
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Function study of EXO70H7 and EXO70H8 genes in Arabidopsis thaliana development.
Modráčková, Jana ; Kubátová, Zdeňka (advisor) ; Soukup, Aleš (referee)
Complex Exocyst consists of eight proteins and it is known as a Sec6/8. Its composition is evolutionarily highly conserved amongst all the species. This complex is involved in vesicle trafficking as a part of attaching mechanism to a specific place on the plasma membrane. EXO70 subunit has been found in 23 copies in Arabidopsis thaliana genome. In this study we have been examine paraloges EXO70H7 and EXO70H8. There have been suggestions that these genes are important in development of roots according to the previous studies. We have not been able to identify any significant phenotype within the mutant plants in these genes. There has been studied other mutant appearance during the stress experiments. Most of these experiments did not identify any divergence. Only experiments with germination during stress conditions revealed significantly worse germination of exo70H7 mutant seeds on the medium containing sorbitol. This suggests that mutant seeds have a worse protection against osmotic stress. Significantly worse germination of exo70H8 seeds on the medium with excess NaCl indicates that these seeds incriminate to higher absorption of sodium ions. Analysis of the cell localization of GFP constructs brought knowledge of appearance EXO70H7 and EXO70H8 proteins. EXO70H7 locates in the cytoplasm and in...
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Mechanisms and regulation of callose deposition to the cell wall of Arabidopsis thaliana
Modráčková, Jana ; Kulich, Ivan (advisor) ; Šustr, Marek (referee)
Callose (β-1,3-glucan) is a significant plant cell wall polysaccharide, which participates in many developmental and stress responses. Despite the importance of this polymer, the knowledge on the causes of callose synthesis, its regulation and the meaning of callose deposition are still limited. The family of callose synthases was revealed thanks to molecular and genetic methods. They are responsible for callose deposition in diferent reactions and the diferent spaces of the plant body. This thesis summarizes the knowleadge about the processes, in which callose is involved, and it presents an overview of the individual proteins from callose synthase family including their phylogenetic analysis and the comparison with the cellulose synthase focusing on the model plant Arabidopsis thaliana. Key words: Arabidopsis thaliana, β-1,3-glucan, callose, callose synthase, plant cell wall polymer
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