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Fluorescence probes in investigation of associative colloid domains.
Mischingerová, Monika ; Krouská, Jitka (referee) ; Mravec, Filip (advisor)
The aim of this thesis was to determine how the concentration of the fluorescent probe (pyrene) affects the critical micelle concentration. In this thesis were used all types of surfactants (anionic, cationic and nonionic). As representatives of the anionic surfactants were chosen sodium dodecylsulfate (SDS) and sodium dodecylbenzenesulfate (SDBS), as the cationic surfactant was chosen cetyltrimethylamonium bromide (CTAB) and as the nonionic polyoxyethylene(9.5)octylphenol (Triton X–100). The critical micelle concentration of these surfactants were measured with a pyrene at three different concentrations. All data obtained by fluorescence spectroscopy were fitted by a Boltzmann-type sigmoid. It was found out that in the case of the anionic surfactants there is some correlation between the critical micelle concentration of the surfactant and the concentration of the fluorescent probe. In both cases (SDS, SDBS) the value of the critical micelle concentration increased with increasing concentration of the fluorescent probe. For the other surfactants (cationic and nonionic) was observed no change in the critical micelle concentration values depending on the concentration of pyrene.
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The penetration features of the hydrofobized hyaluronic acid – based polymeric micelles.
Mischingerová, Monika ; Pekař, Miloslav (referee) ; Muthný, Tomáš (advisor)
The aim of this thesis was to investigate the penetration features of the hydrofobized hyaluronic acid – based polymeric micelles using Nile red as carried tracer. Furthermore, to implement basic characterization of polymeric micelles for potential cosmetic applications using Coenzyme Q10 (CoQ10) as carried substance. It was found that the size of the polymeric micelles with carried CoQ10 did not exceed 100 nm. Applied delivery systems based on hydrophobic hyaluronic acid were suitable for potential topical application. Delivery systems with Nile Red as carried tracer demonstrated excellent penetration features. We assume that delivery systems with CoQ10 will exhibit similar penetration features. An issue has appeared whether the carrier breaks or proceeds along with NR to the skin. Moreover, another experiments have been designed which could also verify the penetration features of these systems.
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The penetration features of the hydrofobized hyaluronic acid – based polymeric micelles.
Mischingerová, Monika ; Pekař, Miloslav (referee) ; Muthný, Tomáš (advisor)
The aim of this thesis was to investigate the penetration features of the hydrofobized hyaluronic acid – based polymeric micelles using Nile red as carried tracer. Furthermore, to implement basic characterization of polymeric micelles for potential cosmetic applications using Coenzyme Q10 (CoQ10) as carried substance. It was found that the size of the polymeric micelles with carried CoQ10 did not exceed 100 nm. Applied delivery systems based on hydrophobic hyaluronic acid were suitable for potential topical application. Delivery systems with Nile Red as carried tracer demonstrated excellent penetration features. We assume that delivery systems with CoQ10 will exhibit similar penetration features. An issue has appeared whether the carrier breaks or proceeds along with NR to the skin. Moreover, another experiments have been designed which could also verify the penetration features of these systems.
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Fluorescence probes in investigation of associative colloid domains.
Mischingerová, Monika ; Krouská, Jitka (referee) ; Mravec, Filip (advisor)
The aim of this thesis was to determine how the concentration of the fluorescent probe (pyrene) affects the critical micelle concentration. In this thesis were used all types of surfactants (anionic, cationic and nonionic). As representatives of the anionic surfactants were chosen sodium dodecylsulfate (SDS) and sodium dodecylbenzenesulfate (SDBS), as the cationic surfactant was chosen cetyltrimethylamonium bromide (CTAB) and as the nonionic polyoxyethylene(9.5)octylphenol (Triton X–100). The critical micelle concentration of these surfactants were measured with a pyrene at three different concentrations. All data obtained by fluorescence spectroscopy were fitted by a Boltzmann-type sigmoid. It was found out that in the case of the anionic surfactants there is some correlation between the critical micelle concentration of the surfactant and the concentration of the fluorescent probe. In both cases (SDS, SDBS) the value of the critical micelle concentration increased with increasing concentration of the fluorescent probe. For the other surfactants (cationic and nonionic) was observed no change in the critical micelle concentration values depending on the concentration of pyrene.
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