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Effect of sunitinib on the expression of iNOS in normotensive and hypertensive rats
Mertlíková, Veronika ; Nachtigal, Petr (advisor) ; Fikrová, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Mgr. Veronika Mertlíková Consultant: Doc. PharmDr. Petr Nachtigal, Ph.D. Title of rigorous thesis: Effect of sunitinib on the expression of iNOS in normotensive and hypertensive rats. The aim of the thesis is to describe the expression of iNOS in the vessel wall of spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats after long-term administration of cytostatic sunitinib. As an experimental model inbred males of SHR rats and normotensive WKY rats were used. They were divided into 2 groups - experimental and control. Sunitinib in drinking water (10mg/ kg per day) was administered to experimental group of rats while the control group of rats recieved only drinking water. In the experimental group of SHR rats, sunitinib was administered for 8 weeks and then after 5 days break it was administered for next 8 weeks. In the experimental group of WKY rats, sunitinib was administered for 8 weeks and then after 5 days break it was administered for only 2 weeks due to weight loss and toxicity symptoms. Immunohistochemical methods were used for detection of iNOS expression, specifically the ABC method. No significant differences of iNOS expression in the...
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The study of VCAM-1 expression in experimental atherogenesis
Mertlíková, Veronika ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Veronika Mertlíková Supervisor: Doc. PharmDr. Petr Nachtigal Ph.D. Title of diploma thesis: The study of VCAM-1 expression in experimental atherogenesis. The aim of the thesis is to describe the expression of VCAM-1 in the vessel wall of normocholesterolemic C57BL/6J mice, which were fed standard laboratory diet, and hypercholesterolemic apoE-deficient mice. As an experimental model normocholesterolemic C57BL/6J male mice and apoE-deficient mice at the age of 6 weeks were used. The C57BL/6J male mice were fed standard laboratory diet and apoE-deficient mice were fed cholesterol diet for 8 weeks. After this procedure a biochemical analysis of blood and immunohistochemical analysis of segments taken from aortic sinus and aortal arch were made. In comparison with the control C57BL/6J mice a large level of cholesterol was found in the blood of the apoE-deficient mice. Atherosclerotic lesions are thus generated only by the hypercholesterolemic mice. We were monitoring the expression of VCAM-1 in aortas of the normocholesterolemic C57BL/6J mice without atherosclerotic lesions by immunohistochemical methods, and the same methods were used for monitoring of the areas with and without...
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Effect of sunitinib on the expression of iNOS in normotensive and hypertensive rats
Mertlíková, Veronika ; Nachtigal, Petr (advisor) ; Fikrová, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Mgr. Veronika Mertlíková Consultant: Doc. PharmDr. Petr Nachtigal, Ph.D. Title of rigorous thesis: Effect of sunitinib on the expression of iNOS in normotensive and hypertensive rats. The aim of the thesis is to describe the expression of iNOS in the vessel wall of spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats after long-term administration of cytostatic sunitinib. As an experimental model inbred males of SHR rats and normotensive WKY rats were used. They were divided into 2 groups - experimental and control. Sunitinib in drinking water (10mg/ kg per day) was administered to experimental group of rats while the control group of rats recieved only drinking water. In the experimental group of SHR rats, sunitinib was administered for 8 weeks and then after 5 days break it was administered for next 8 weeks. In the experimental group of WKY rats, sunitinib was administered for 8 weeks and then after 5 days break it was administered for only 2 weeks due to weight loss and toxicity symptoms. Immunohistochemical methods were used for detection of iNOS expression, specifically the ABC method. No significant differences of iNOS expression in the...
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The study of VCAM-1 expression in experimental atherogenesis
Mertlíková, Veronika ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Veronika Mertlíková Supervisor: Doc. PharmDr. Petr Nachtigal Ph.D. Title of diploma thesis: The study of VCAM-1 expression in experimental atherogenesis. The aim of the thesis is to describe the expression of VCAM-1 in the vessel wall of normocholesterolemic C57BL/6J mice, which were fed standard laboratory diet, and hypercholesterolemic apoE-deficient mice. As an experimental model normocholesterolemic C57BL/6J male mice and apoE-deficient mice at the age of 6 weeks were used. The C57BL/6J male mice were fed standard laboratory diet and apoE-deficient mice were fed cholesterol diet for 8 weeks. After this procedure a biochemical analysis of blood and immunohistochemical analysis of segments taken from aortic sinus and aortal arch were made. In comparison with the control C57BL/6J mice a large level of cholesterol was found in the blood of the apoE-deficient mice. Atherosclerotic lesions are thus generated only by the hypercholesterolemic mice. We were monitoring the expression of VCAM-1 in aortas of the normocholesterolemic C57BL/6J mice without atherosclerotic lesions by immunohistochemical methods, and the same methods were used for monitoring of the areas with and without...
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