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Data Backup and DataStorage
Markovič, Adam ; Šustrová, Tereza (referee) ; Kříž, Jiří (advisor)
The bachelor thesis deals with the issue of data storage and related data backup. The thesis is divided into several parts, which are all focused on solving the problem of data backup in XZ company. The result of the bachelor thesis is the actual solution proposal, which is based on the theoretical part and the analysis of the current state of backup in the company.
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Subunit c of mammalian F1Fo ATP synthase - from molecular mechanisms of assembly to potential therapies
Marković, Aleksandra ; Mráček, Tomáš (advisor) ; Trnka, Jan (referee) ; Rohlena, Jakub (referee)
Mammalian F1Fo ATP synthase plays a crucial role in ATP production through the process of oxidative phosphorylation. Assembly of this multisubunit protein complex requires specific assembly factors. Notably, the assembling of subunit c in mammals requires several factors, yet despite decades of research, the import of subunit c, its incorporation into the inner mitochondrial membrane, and the assembly of monomers towards octameric c-ring remain enigmatic. To shed new light on this process, we first screened for interacting partners of subunit c using a mass spectrometry-based approach. Our screen identified three proteins as the most prominent interactors of subunit c- TMEM70, TMEM242, and c15orf61, which we subsequently characterized. For the initial characterization of the TMEM242 function, we generated both knockdown and knockout HEK293 models. TMEM242 knockdown led to impaired biogenesis and decreased levels of assembled ATP synthase without affecting the content of other OXPHOS complexes. On the contrary, complete deficiency of TMEM242 lead also to the downregulation of complexes I and IV, which indicates that the primary target of TMEM242 is ATP synthase. While other studies suggested that both TMEM242 and TMEM70 interact with mitochondria complex I intermediate assembly (MCIA), our...
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Data Backup and DataStorage
Markovič, Adam ; Šustrová, Tereza (referee) ; Kříž, Jiří (advisor)
The bachelor thesis deals with the issue of data storage and related data backup. The thesis is divided into several parts, which are all focused on solving the problem of data backup in XZ company. The result of the bachelor thesis is the actual solution proposal, which is based on the theoretical part and the analysis of the current state of backup in the company.
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