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Transport of selected drugs in the renal cells
Lysá, Tereza ; Trejtnar, František (advisor) ; Melicharová, Ludmila (referee)
7. ABSTRACT Somatostatin analogues labelled with radionuclides are used for diagnosis and therapy of some tumours, mainly neuroendocrine ones, in which receptors specifically binding these radiolabelled analogues of somatostatin are occurred. However, the therapeutic usage of these radiolabelled analogues of somatostatin is limited due to nefrotoxic undesirable effects originated because of their accumulation in the renal proximal tubules after reabsorption from the glomerular filtrate. The aim of the study was to contribute to knowledge in the renal transport systems responsible for reabsorption of radiolabelled somatostatin analogues in the renal tubular cells and then on the base of such data to find some substances which could reduce the renal accumulation and prevent the damage of the renal tissue. In experiments, the isolated renal cells obtained from rat kidney by collagenase method were used. After the verification of presence and function of transport mechanism by known indicators of active and passive transport, the accumulation of two somatatostatin analogues labelled with radionuclides - 111In-DOTA-NOC and 125IDOTA- Tyr3-octreotate were studied on these renal cells. The active transport of these radiopeptides was verified by reduce of incubation temperature. Then changes of accumulation of these...
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Transport of selected drugs in the renal cells
Lysá, Tereza ; Trejtnar, František (advisor) ; Melicharová, Ludmila (referee)
7. ABSTRACT Somatostatin analogues labelled with radionuclides are used for diagnosis and therapy of some tumours, mainly neuroendocrine ones, in which receptors specifically binding these radiolabelled analogues of somatostatin are occurred. However, the therapeutic usage of these radiolabelled analogues of somatostatin is limited due to nefrotoxic undesirable effects originated because of their accumulation in the renal proximal tubules after reabsorption from the glomerular filtrate. The aim of the study was to contribute to knowledge in the renal transport systems responsible for reabsorption of radiolabelled somatostatin analogues in the renal tubular cells and then on the base of such data to find some substances which could reduce the renal accumulation and prevent the damage of the renal tissue. In experiments, the isolated renal cells obtained from rat kidney by collagenase method were used. After the verification of presence and function of transport mechanism by known indicators of active and passive transport, the accumulation of two somatatostatin analogues labelled with radionuclides - 111In-DOTA-NOC and 125IDOTA- Tyr3-octreotate were studied on these renal cells. The active transport of these radiopeptides was verified by reduce of incubation temperature. Then changes of accumulation of these...
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Incidence of Alcoholism of the University Students
LYSÁ, Tereza
My bachelor thesis deals with abusing alcohol by university students. It mainly tracks the impact of alcohol on student lifestyle, on behavior acompaining its use and how it affects students attending different specialisations. The thesis consists of two parts. Comparison of opinions on addictions, alcohol and youth lifestyle questions based on available literature research and sources research is put across in the first, theoretical, part. In second, practital, part I treat with field research plan and targets and interpretation of acquired outcomes. Survey was realized between april 2010 ? february 2011.
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