Home > Search Results: Lopatářová, Miloslava
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Gene expression during oocyte-cumulus expansion and preimplantation embryo development in mammals
 Němcová, Lucie ; Kaňka, Jiří (advisor) ; Petr, Jaroslav (referee) ; Lopatářová, Miloslava (referee)
CONCLUSIONS Major resultsof this PhD thesiscan be summarizedas follows: The real-time RT-PCR has become a tool for detection and quantifrcation of RNA targetsandis being utilized in geneexpressionstudyin our laboratory. The gene expression proÍile of GDF9, during porcine oocytes, cumulus andgranulosacells fu vitro cultivation and in vivo cumulus oocyte expansion was assessed.The obtained partial sequence was up|oaded in EMBL/GenBanlďDDBJ databaseand gene was localized on pig chromosome2. Conhary to mouse,GDFS mRNA was detectedfor the ťrrsttime also in porcine somaticfollicular cells. During cultivation,thetranscriptgraduallydecreasedin all the samples. we studied signalling pathways involved in IGFI promotion of FSH-induced oocyte-cumulusexpansion.we found that IGFI in combinationwith FSH increased the production of HA. The expression of Has2 mRNA in cumulus cells gradually decreasedduring cultivation' our results suggest that the PIK3/AKT dépendent pathwayis involved in the promotionof FSH stimulatedsynthesisof HA in porcine OCCs. Bovine blastocystsdeveloped from fertilized oocýes under different in vitro or in vivo conditions were comparedin the expressionof threegenes(Bax, L37 and S3o/.The embryosdiffer in the abundanceof Bax transcripl the elevatedlevels were detected in blastocyst completely cultivated...
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English