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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Using vibrational spectroscopy for mapping of NOS active site
 Lidický, Ondřej ; Hudeček, Jiří (advisor) ; Martínek, Václav (referee)
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Using vibrational spectroscopy for mapping of NOS active site
 Lidický, Ondřej ; Martínek, Václav (referee) ; Hudeček, Jiří (advisor)
Detailed record
Heterologous expression of the E7 oncoprotein from human papillomavirus HVP16
 Lidický, Ondřej ; Hudeček, Jiří (advisor) ; Mach, Otakar (referee)
Production of vaccines and pharmaceutical proteins in plants is a promising nascent technology with a great potential to provide high-quality, safe and non-expensive production and delivery platform. In this work we studied the experimental vaccine against human papillomavirus based on modified plant pathogen - Potato virus X (PVX). The experimental vaccine is based on PVX virus particles decorated with genetically fused HPV-E7 oncoprotein. These chimeric virus particles should be able to activate strong and specific cellular immune response. However the modification of the PVX coat protein with such relatively large fused protein might influence its ability to form particles. In this work we have characterized some properties of such chimeric virus particles like solubility or ability infect host plant. (In Czech)
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