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Analysis of cough-cold preparations by electrophoretic methods.
Kubíková, Drahomíra ; Pospíšilová, Marie (advisor) ; Jáč, Pavel (referee)
In this study, micellar elektrokinetic chromatography method (MEKC) was developed for the determination of paracetamol (PA), caffein (CO), ascorbic acid (AA) and phenylephrine (FE) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (31,2 cm x 50 m i.d. effective length 21 cm) at 20 kV with UV detection at 190 nm and 265 nm. Optimized background electrolyte (BGE) was 100 mM boric acid containing 100 mM sodium dodecyl sulfate (SDS) as a surfactant and 10 % (v/v) of methanol. pH* 7,8 was found to be optimal (modified by 50 mM sodium tetraborate). Methylparaben (MP) was used as internal standard. This validated method has been successfully applied for the analysis of commercially available pharmaceutical product (Coldrex tbl.).
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Determination of Paracetamol, Ascorbic Acid and Phenylephrine in Pharmaceutical Preparations by Micellar Electrokinetic Chromatography
Kubíková, Drahomíra ; Polášek, Miroslav (referee) ; Pospíšilová, Marie (advisor)
DETERMINATION OF PARACETAMOL, ASCORBIC ACID AND PHENYLEPHRINE IN PHARMACEUTICAL PREPARATIONS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY Author: Kubikova D. Tutor: Pospisilova M., Dept. of Analytical Chemistry, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague A new method of micellar elektrokinetic chromatography (MEKC) with UV detection at 190 nm and 265 nm for the determination of paracetamol (PA), ascorbic acid (AA) and phenylephrine (FE) in pharmaceutical preparations was devised and optimised. The analysis was carried out in a fused-silica capillary (internal diameter 50 m, total length31,2 cm and effective length 21 cm), with voltage 20 kV, temperature 20 o C and sample injection for 5 seconds under the pressure of 34,5 mbar. Optimum background electrolyte (BGE) was 5 mM sodium tetraborate and 5 mM potassium dihydrogen phosphate containing 150 mM sodium dodecyl sulphate (SDS) as a surfactant and 10 % (v/v) of methanol. pH* 8,2 was found to be optimal. Methylparaben (MP) was used as internal standard. The peaks of the drugs were satisfactorily separated from the peak of internal standard as well as from the EOF. The separation took less than 9 minutes and the overall analysis time involving appropriate rinsing of the capillary was less than 15 minutes. The calibration curves were...
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Analysis of cough-cold preparations by electrophoretic methods.
Kubíková, Drahomíra ; Jáč, Pavel (referee) ; Pospíšilová, Marie (advisor)
In this study, micellar elektrokinetic chromatography method (MEKC) was developed for the determination of paracetamol (PA), caffein (CO), ascorbic acid (AA) and phenylephrine (FE) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (31,2 cm x 50 m i.d. effective length 21 cm) at 20 kV with UV detection at 190 nm and 265 nm. Optimized background electrolyte (BGE) was 100 mM boric acid containing 100 mM sodium dodecyl sulfate (SDS) as a surfactant and 10 % (v/v) of methanol. pH* 7,8 was found to be optimal (modified by 50 mM sodium tetraborate). Methylparaben (MP) was used as internal standard. This validated method has been successfully applied for the analysis of commercially available pharmaceutical product (Coldrex tbl.).
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