|
|
Effect of cancer therapy on fertilization parameters in testicular germ cell tumour patients.
Kruf, Annabelle Elisabeth ; Vondráková, Jana (advisor) ; Nevoral, Jan (referee)
Testicular germ cell tumours (TGCTs) are a rare type of cancer. It is the most common type of cancer in the age group of 15-35 years. The consequences that TGCT and the treatment of this disease impose to these young men in terms of sperm parameters and fertility is yet unclear. This study involves 84 semen samples from 46 patients sorted in three time groups - before treatment, 3 months after the treatment and 6 months after the treatment. Several sperm parameters were studied and compared within the three time points. Adding on to this, the study takes two patients with complete set of semen samples and studies them individually. The results mostly show great differences in patients and this study underlines the individual characteristics that need to be taken into consideration when it comes to patients' fertility journeys after battling TGCT. Sperm viability, concentration, apoptosis, DNA damage, acrosomal integrity and leukocyte concentration nor histone modification evaluation showed any significant changes between said groups. DNA damage was correlated to the level of acrosomal permeability. IgA antibody levels rose significantly in samples after orchiectomy. IgG antibody levels did not show the same trend. A novel approach was optimized and applied using JUNO and FcRL3 double transfected...
|
|
|
Testicular germ cell tumor related sperm and testis germ cell pathologies: experimental and clinical angle.
Kruf, Annabelle Elisabeth ; Svobodová, Jana (advisor) ; Porubská, Bianka (referee)
This bachelor thesis focuses on testicular germ cell tumors (TGCT) and associated sperm and tissue pathologies in the context of different clinical and experimental perspectives. Testicular cancer is the most common malignancy among young men in the reproductive age and the worldwide incidence of testicular cancer is on the rise. This thesis emphasizes the importance of better cooperation between scientific findings from primary research and their clinical implementations in cancer management. The main aim of this thesis is not only to summarize current knowledge of healthy and tumor affected sperm and testes tissue, but primarily define clinical and especially experimental approaches of how to reveal, study and treat TGCT. In order to gain a deeper understanding and insight into TGCT, the data discussed in this thesis comes from the genetic, epigenetic, proteomic, endocrinologic and metabolic fields. More research on the underlying mechanisms of testicular cancer will further improve the quality of life of many young people faced with this diagnose. Keywords: sperm parametrs, testicular germ cells, testicular tumors, tumor treatment, diagnostic strategies
|
guest ::
