|
|
Immunohistochemical analysis of the expression of markers in experimental hypertension.
Krejčíková, Veronika ; Nachtigal, Petr (advisor) ; Kudláčková, Zděnka (referee)
Veronika Krejčíková Immunohistochemical analysis of the expression of markers in experimental hypertension. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Background: We observed the expression of intercellular adhesion molecule (ICAM-1) in the right femur arteries of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats with regard to the administration of sunitinib. Methods: We used the male of SHR and WKY rats. Each strain of the rats was divided into two groups. Each of groups were divided into two subset. One of subset received sunitinib and the second, control subset, which received only water. SHR were treated by sunitinib in the following chart: 8 weeks treatment/5 weeks pause/8weeks treatment. WKY rats were treated by sunitinib only in fhe following chart: 8 weeks treatment/ 5 weeks pause/8 weeks treatment, because signs of toxicity were appeared. After that immunohistochemical analysis of segments of right femur arteries were executed. Detection of expression of ICAM-1 was performed using the method of En Vision with detection using DAB. Results: We performed the immunohistochemical detection of ICAM-1 in 120 slides. In the some of them we found no expression of ICAM-1 regardless to subset of testing animals....
|
|
|
The effect of GLP-1 (glucagon-like peptide-1) analogues on rat hepatocytes in vitro
Krejčíková, Veronika ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Bc. Veronika Krejčíková The effect of GLP-1 (glucagon-like peptide-1) analogues on rat hepatocytes in vitro. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in Laboratory Methods Background: We observed the effect of liraglutide (LIRA; a GLP-1 analogue) on rat non-steatotic (ST-1) and steatotic (HF) rat hepatocytes in vitro. We also monitored its potential hepatoprotective effect on the cells damaged by acetaminophen (APAP). Methods: As a model for our experiments we used the primary cultures of rat hepatocytes isolated from the liver of male Wistar rats. For isolation of lean and fatty hepatocytes, we used rats fed by standard laboratory diet (10% energy from fat) and high fat diet (71% energy from fat) for 6 weeks. Hepatocytes were isolated by two-step collagenase perfusion. We used LIRA at concentrations of 1, 10 and 100 nmol/l on intact, ST-1 and HF hepatocytes; we also tested effect of LIRA on hepatocytes after exposure to 2.5; 3.75 and 5 mM APAP. After 24/20-hours incubation, we tested the activity of lactatdehydrogenase (LDH) in culture medium, the viability of hepatocytes, production of albumin, and level of MDA and ROS production. Morphological evaluation of cells was performed by using phase microscopy. Results: LIRA at tested concentrations...
|
|
|
Immunohistochemical analysis of the expression of markers in experimental hypertension.
Krejčíková, Veronika ; Nachtigal, Petr (advisor) ; Kudláčková, Zděnka (referee)
Veronika Krejčíková Immunohistochemical analysis of the expression of markers in experimental hypertension. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Background: We observed the expression of intercellular adhesion molecule (ICAM-1) in the right femur arteries of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats with regard to the administration of sunitinib. Methods: We used the male of SHR and WKY rats. Each strain of the rats was divided into two groups. Each of groups were divided into two subset. One of subset received sunitinib and the second, control subset, which received only water. SHR were treated by sunitinib in the following chart: 8 weeks treatment/5 weeks pause/8weeks treatment. WKY rats were treated by sunitinib only in fhe following chart: 8 weeks treatment/ 5 weeks pause/8 weeks treatment, because signs of toxicity were appeared. After that immunohistochemical analysis of segments of right femur arteries were executed. Detection of expression of ICAM-1 was performed using the method of En Vision with detection using DAB. Results: We performed the immunohistochemical detection of ICAM-1 in 120 slides. In the some of them we found no expression of ICAM-1 regardless to subset of testing animals....
|
guest ::
