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New psychotropic drugs and their effect on developing embryo
Košťalová, Jana ; Maňáková, Eva (advisor)
In my diploma thesis, I researched the effect of mirtazapine on a developing embryo. Nowadays, new drugs are being developed and used for the treatment of depression. These drugs also include mirtazapine. The risk of using mirtazapine during pregnancy has not been documented yet and therefore it can not be used by pregnant women. These drugs are often very effective and well tolerated so it is very important to recognize the effect on a developing embryo. In our research, we used the method of CHEST (chick embryotoxicity screening test). We applied a dose of mirtazapine solution dissolved in DMSO to 4-day old chickens. The doses were 0.2µg, 0.15µg, 0.1µg, 0.05µg, 0.03µg in 3µl of the solution. As controls, we applied 3µl DMSO and 3µl destilled water. Embryos were being incubated for 5 days in an incubator and on the 9th day, we evaluated dead and malformed embryos and the spectrum of defects. From our observations, we have obtained the lethal dose, which was above 0.15µg and the dose that was equivalent to the LD50 - 0.05µg. Lower doses were safe, although these embryos were malformed. These malformations, however, were not statistically significantly different from the malformations occurring in controls. It is neccessary to continue and complete data for 2 -and 3-day old embryos, so that we cover all...
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Liability for damage caused by unlawful decisions or maladministration
Košťálová, Jana ; Pohl, Tomáš (advisor) ; Macková, Alena (referee)
Liability for damage caused by unlawful decision or maladministration The concept of liability for damage is one of the basic institutions of private and public law. Civil liability for damage provides protection for property and personal relations of natural persons and legal entities. Liability for damage is formed by a fulfilment of conditions stipulated by law. The aim of this liability is to eliminate the consequences of breaking the law and to prevent the occurrence of damage through pre-emption. As a topic of my thesis, I have chosen the specific case of civil liability which is the liability for damage caused by unlawful decision or maladministration. I will specifically focus on the liability of the state for damages caused by execution of public authority, where the state acts as a public entity in a legal relationship. The aim of state liabilty caused by execution of public authority is to provide legal certainty to parties in a legal relationship. If damage is caused as per the conditions defined by law, the aim of state liability for damage i salso remove any unwanted consequences of breaking law. Last but not the least, the function of state liability for damage caused by execution of public authority is to prevent the damages in the first place. In my thesis I provide a comprehensive...
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New psychotropic drugs and their effect on developing embryo
Košťalová, Jana ; Maňáková, Eva (advisor)
In my diploma thesis, I researched the effect of mirtazapine on a developing embryo. Nowadays, new drugs are being developed and used for the treatment of depression. These drugs also include mirtazapine. The risk of using mirtazapine during pregnancy has not been documented yet and therefore it can not be used by pregnant women. These drugs are often very effective and well tolerated so it is very important to recognize the effect on a developing embryo. In our research, we used the method of CHEST (chick embryotoxicity screening test). We applied a dose of mirtazapine solution dissolved in DMSO to 4-day old chickens. The doses were 0.2µg, 0.15µg, 0.1µg, 0.05µg, 0.03µg in 3µl of the solution. As controls, we applied 3µl DMSO and 3µl destilled water. Embryos were being incubated for 5 days in an incubator and on the 9th day, we evaluated dead and malformed embryos and the spectrum of defects. From our observations, we have obtained the lethal dose, which was above 0.15µg and the dose that was equivalent to the LD50 - 0.05µg. Lower doses were safe, although these embryos were malformed. These malformations, however, were not statistically significantly different from the malformations occurring in controls. It is neccessary to continue and complete data for 2 -and 3-day old embryos, so that we cover all...
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