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Evaluation of antibody quality for Endoglin detection in liver
Kejzlarová, Eliška ; Svobodová, Zuzana (advisor) ; Tripská, Katarína (referee)
The diploma thesis deals with endoglin, a transmembrane glycoprotein that can be cleaved to form so-called soluble endoglin, which then circulates in the blood. The theoretical part of the thesis is devoted to cardiovascular and liver diseases related to changes in the expression or level of endoglin in the blood and summarizes current knowledge about endoglin and its relation to various diseases. The experimental part of the thesis describes the selection of a suitable antibody against endoglin intended for western blot (WB). We have four anti-endoglin primary antibodies available from three manufacturers. The purpose was to test the antibodies and determine, which one had the best ability to detect endoglin in mouse tissue samples such as liver or aorta on WB. Affiblot was used to verify their ability to bind endoglin and also to compare their affinities to this protein. Newly developed patented dot-blot-based device. The binding of the used antibodies to endoglin was verified using the WB method and then the results of both methods were compared. The obtained results showed that the Abcam antibody has the most suitable parameters regarding specificity and sensitivity for the identification of endoglin in mouse tissue on WB. Keywords: endoglin, soluble endoglin, NASH, cirrhosis, non-alcoholic...
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Cirkculating tumour cells and their role in breast cancer treatment
Kejzlarová, Eliška ; Svobodová, Zuzana (advisor) ; Vicen, Matej (referee)
The bachelor thesis summarizes the existing knowledge about circulating tumour cells (CTC) and their use in the diagnosis and treatment of breast cancer. First is presented a chapter about cancer in general. The following chapter deals with breast cancer, its etiology including genetic factors and other risk factors for its formation. Furthermore, there are types of breast cancer in women and men and the possibilities of their diagnosis and imaging methods. The next part is devoted to CTC, their definition and role in metastatic and primary breast cancer. Due to their low concentration in the blood of patients, special methods have been developed for their identification and detection. These methods are summarized in the following chapter. The number of CTCs in the blood of patients with breast cancer has a significant prognostic significance in the diagnosis and treatment by doctors, so it is important to monitor the current knowledge so that they can get into the common practice of cancer treatment as soon as possible. Key words: cancer, breast carcinoma, circulating tumour cells, diagnosis
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