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Fytochemical potencial of polyfenols contain in berries
KRUMPHANZLOVÁ, Iva
This thesis sums up findings on polyphenols and their positive influence on human health. In these fytochemicals, unlike in micronutrients, recommended daily amount cannot be estimated. Micronutrient deficiency in food can cause deficiency symptoms, while in polyphenol deficiency, the deficiency does not occur. When they are present in sufficient amount in food, it is considered functional food, which is important for civilization illnesses prevention. At the beginning of the theoretical part, the polyphenol classification from pharmacological and chemical point of view is represented. On the basis of this classification important role of antioxidants and flavonoids is described. Special attention is being paid to resveratrol, which is populardue to its well known "French paradox". The following part considers the polyphenol influence on human health. The way in which polyphenols protect our body from certain diseases is explained in detail. Most of the health benefits are supported by studies conducted on animals. These studies are also mentioned and described in this paper. In the theoretical part the process of absorption and the polyphenols safety for human body is also explained. Polyphenol sources are listed in the last subchapter. The work is mainly aimed at blueberry fruits (Vaccinium myrtillus) and vine grapes (Vitis vinifera). Other berries are briefly mentioned as well, such as cranberries, raspberries, blackberries and currant. The goal of this work is to create a diet that would provide a functional daily amount of polyphenols. This amount can be compared to 120 ml of native blueberries. Only products, consumable without cooking, are used in this diet. The second goal is to chart, which food products are adequate source of polyphenols. On the basis of these goals, research inquiries were defined. Research inquiry No. 1: What amount of polyphenols is contained in berries, especially blueberries. Research inquiry No. 2: Which food products (without cooking) are ideal to be included in diet, to provide adequate daily amount of polyphenols. To asses polyphenol content in blueberries, analysis of polyphenol content acquired through GEOMED program from Nemocnice České Budějovice a.s., department of clinical pharmacology, was used. Assessments were made using the HPLC method (high performance liquid chromatography) in cooperation with the biological centre AV ČR in České Budějovice. Thanks to this analysis, the first inquiry can be answered. An interesting phenomenon is discovered, that frozen blueberries have higher polyphenol content then cooled ones. Malvidin (malvidin-3-6"-acetoyl glycoside) was chosen as priority anthocyan since it is most represented in the blueberry. Remaining information about polyphenol content in certain food products were drawn on Joseph Maroon´s book, MD, FACS (2010). The practical part involves a diet for 14 days, which meets the given requirements. The results clearly show that it is possible to create a diet rich in polyphenols. A list of blueberry products accessible to everyone is included. Blueberry dairy products, jams, beverages, fruit salads and fruit pyre have been analysed. The list shows which product contains the most polyphenols. The required amount can be achieved by the proper combination of given products. The ideal one proved to be 200 ml of red wine and one blueberry dairy product (with the malvidin value higher than 64). Findings in this paper will serve to promote polyphenol awareness and itseffects. This diet can be used in nursing homes, where their positive influence on equilibrium can be applied. It can also be used in spas and other alimentation related facilities.
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