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HPLC Analysis of Vitamines Soluble in Water Using Electrochemical Detector
Jesenský, Ivan ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
High performacne liquid chromatography is valued analytical method, which is one of the most frequently used in pharmacy. Biotine is at water soluble vitamin, which is an important element of various enzyme, stuff necessary in metabolism. It part take on metabolism of sugar, fat and protein. It occur in several sort of food, especially in roast, peanut butter, egg yolk, carrot, peas, soya, wheat gemmule and oaten flake. This work deals with optimalization chromatographic condition on determination of biotine. Its target is to find optimal mobile phase, near which give out best separation biotine from others element in solution, to find internal standard and form hydrodynamic voltamogramms, individual at water soluble vitamin. For determinatiom was used coulometric detector Coulochem III. Analysis overshoot on column Lichrospher 60, RP - select B (240 x 4,0 mm and.d., 5 em), Merck. Mobile phase was composed of acetic acid : acetonitrile (CH3COOH 0,2410 mol/l; pH 3,0) 85: 15. Flow rate was 1ml/min, in temperature 30žC. Current inserted on working electrode was 900 mV, sensitiveness 200mV.
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HPLC Analysis of Vitamines Soluble in Water Using Electrochemical Detector
Jesenský, Ivan ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
High performacne liquid chromatography is valued analytical method, which is one of the most frequently used in pharmacy. Biotine is at water soluble vitamin, which is an important element of various enzyme, stuff necessary in metabolism. It part take on metabolism of sugar, fat and protein. It occur in several sort of food, especially in roast, peanut butter, egg yolk, carrot, peas, soya, wheat gemmule and oaten flake. This work deals with optimalization chromatographic condition on determination of biotine. Its target is to find optimal mobile phase, near which give out best separation biotine from others element in solution, to find internal standard and form hydrodynamic voltamogramms, individual at water soluble vitamin. For determinatiom was used coulometric detector Coulochem III. Analysis overshoot on column Lichrospher 60, RP - select B (240 x 4,0 mm and.d., 5 em), Merck. Mobile phase was composed of acetic acid : acetonitrile (CH3COOH 0,2410 mol/l; pH 3,0) 85: 15. Flow rate was 1ml/min, in temperature 30žC. Current inserted on working electrode was 900 mV, sensitiveness 200mV.
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