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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Effect of adaptive mutations on persister formation in Staphylococcus aureus
 Hloucalová, Nikola ; Lichá, Irena (advisor) ; Buriánková, Karolina (referee)
Persistence represents a transient state during which are bacterial cells able to survive antibiotic treatment. Only a small subpopulation of cells enter this state, these cells are then capable of causing disease recurrence. The size of a persister subpopulation is influenced by adaptive mutations formed during a chronic disease in genes related to transition of cells between a virulent form and biofilm formation. Regulation of entering and exiting persistence is also influenced by intercellular communication and by molecules produced into the cell surroundings. It is assumed that this effect is caused by PSM modulines and AIP molecules which take part in Quorum sensing mechanism. The main aim of this thesis was to clarify how adaptive mutations of clinical isolates obtained from two patients diagnosed with cystic fibrosis influence the ability to persist. Another aim was to determine changes in membrane potential and metabolic aktivity while entering persistence and to establish the effect of extracellular molecules produced into the culture medium on growth parameters of the studied isolates. By using techniques of TD test and establishment of persistence rate by CFU determination, it was found that the isolates obtained from patient 2 did not acquire adaptive mutations affecting persistence...
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The effect of antibiotics on human gut microbiome and the influence of probiotics on its restoration
 Hloucalová, Nikola ; Lichá, Irena (advisor) ; Ulrych, Aleš (referee)
Antibiotics are used for treatment of bacterial infections. They negatively affect not only the pathogens, but also other microorganisms in the gut, including the beneficial bacteria. Antibiotic treatment changes the proportion of good versus bad bacteria in the gut, causes a decrease in the number of commensal bacteria and leads to the overgrowth of opportunistic pathogens. We should consume probiotics during and after the antibiotic treatment, otherwise it results in an unhealthy stool and moreover it affects the immune system which then leads to physical and mental illnesses. This thesis summarizes the influence of probiotics on human gut during dysbiosis caused mainly by antibiotics.
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