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Analysis and characterization of antimicrobial peptides by capillary electrophoresis
Hlavsová, Tereza ; Coufal, Pavel (advisor) ; Čabala, Radomír (referee)
Capillary zone electrophoresis (CZE) with UV-photometric detection at 206 nm was used for the determination of degree of chemical purity, limit of detection and limit of quantitation of antimicrobial cationic peptide halictine and its 25 analogs. Halictine and its analogs were chracterized by their effective electrophoretic mobilities corrected to reference temperature 25 řC in several acidic background electrolytes. Separations of mixtures of structurally related analogs of halictine were performed by capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC). The highest values of separation effeciency, up to 2.36∙105 theoretical plates per meter, were achieved by CZE in the background elektrolyte containing 30 mM phosphoric acid, 25 mM Tris and additive of cationic surfactant 0.4 mM didodecyldimethylammonium bromide (DDAB), pH 2.85. The highest values of resolution of CZE separations of peptides were found in background electrolyte composed of 30 mM phosphoric acid, 25 mM Tris, 0.4 mM DDAB and 0.1 % (m/v) hydroxyethylcellulose, pH 2.84.
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Preschool children and theatre performance
HLAVSOVÁ, Tereza
This bachelor's thesis examines the preschool children and theatre performance. The aim of this work is discover whether children benefit more from a theatre performance they themselves playact in, or from one which they watch. The thesis also focuses on behaviour and dressing of children in theatre premises. The theoretical section of the work focuses on definition of the term 'drama' and it's various parts. Further, it examines emergence and history of drama and dramatic guilds. In this section, drama is subdivided into two parts: acted theatre and puppet theatre. The practical section uses observational and conversational research methods to investigate reactions and behaviour of children during a performance, which they either take part in or watch. It further focuses on child behaviour in the premises of kindergarten and theatre, where the performance takes place.
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