|
|
Signs of epilepsy spectrum disorder in a student sample of non-smokers and smokers
Gregorová, Klaudie ; Herink, Josef (advisor) ; Kudláčková, Zděnka (referee)
v anglickém jazyce Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Klaudie Gregorová Supervisor: Doc. MUDr. Josef Herink, DrSc. Title of diploma thesis: Signs of epilepsy spectrum disorder in a student sample of non- smokers and smokers The diploma thesis deals with signs of epilepsy spectrum disorder in a student sample of non-smokers and smokers and it continues in thesis of the author M. Světlák and of the author P. Zitová. The diploma thesis is divided into the two main parts - the theoretical part summarized the issue of the temporo-limbic dysfunction, the research part answers the put questions. The main goal of the diploma thesis was to describe the issue of the temporo-limbic dysfunction in the university student sample divided into smokers and non-smokers.
|
|
|
Temporolimbic dysfunction in persons with sleep disorders
Hepnerová, Eva ; Herink, Josef (advisor) ; Kudláčková, Zděnka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medicinal Sciences Student: Bc. Eva Hepnerová Supervisor: Doc. MUDr. Josef Herink, DrSc. Title: Temporolimbic dysfunction in persons with sleep disorders Background: The aim is to demonstrate the occurrence of symptoms of the temporolimbic dysfunction in person with sleep disorders using standard psychosocial questionnaires. Methods: The occurrence of symptoms of the temporolimbic dysfunction was detected by means of CPSI and LSCL-33 questionnaires. Correlation of both questionnaires' results was evaluated by Spearman's coefficient. Results: According to CPSI 11 % patients have abnormal score and they have higher occurrence of symptoms of the temporolimbic dysfunction. According to LSCL-33 achieve 32 % of respondents abnormal score and 7 % respondents have pathologic score, which means the presence of the temporolimbic dysfunction. Conclusions: The occurrence of the temporolimbic dysfunction in persons with sleep disorders have been shown, but it haven't been higher in comparison with healthy population. Keywords: mood disorders, sleep disorders, psychosocial questionnaire, sleep, temporolimbic region
|
|
|
Drug induced imbalance of sodium
Šteflová, Iveta ; Herink, Josef (advisor) ; Pourová, Jana (referee)
Iveta Šteflová Drug induced imbalance of sodium Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Department of Biological and Medical Science Supervisor: Doc.MUDr. Josef Herink, DrSc. Sodium (Na+ ) is the major extracellular cation. It plays an important role in maintaining membrane potential and depolarization that is the basic mechanism of transmission of the nerve impulse. It is involved in maintaining acid-base balance, osmotic pressure, water retention in the body. The largest part of the sodium is in the extracellular fluid where it is stored about 50 % of sodium. Plasma concentration of sodium is 140 ± 5 mmol/l. Drug-induced electrolyte disorders are increasingly reported and may be associated with considerable morbidity and mortality. The risk of drug-induced hyponatremia is generally higher than the risk of drug-induced hypernatremia. Hyponatremia is a common electrolyte disorder defined as a decrease plasma sodium concentration below 135 mmol/l. It is classified by the state of volume - hypovolemic, euvolemic and hypervolemic hyponatremia. It reflects the relative rate between sodium and water in the body. The most common cause is the syndrome of inappropriate secretion of antidiuretic hormone that induces euvolemic hyponatremia. Hypernatremia is...
|
|
|
Testing the efficacy of potential therapeutics for Alzheimer,s disease
Dolejšová, Adéla ; Herink, Josef (advisor) ; Vopršalová, Marie (referee)
Adéla Dolejšová Testing the efficacy of potential therapeutics for Alzheimer,s disease Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Pharmacy The aim of this thesis was to find out whether already implemented Ellman's method, which is used to analyse irreversible inhibitors AChE, is going to be a suitable technique for measuring reversible inhibitors. Furthermore, the efficiency of newly synthesized AChE inhibitors was established. These inhibitors will be used for treating AD or as prophylaxis against neural paralytic substances. Moreover, it was compared affect the efficacy of AChE inhibitors after intramuscular and intraperitoneal administration. The measurements were done in vivo on potkan species Wistar. In the first experiment standard AChE (tacrin, 7-MEOTA, donepezil, rivastigmin) inhibitors were applied to verify the method. In the second experiment the newly synthesizes AChE (K 298, K 344, K 474) inhibitors were medicine. The results confirmed the effectiveness of the tested method on commonly used inhibitors such as donepezil and rivastigmin. Out of the newly synthesized K 298, K 344 and K 474 inhibitors none was proven to have any significant inhibiting activity. Key words: acetylcholinesterase, acetylcholinesterase inhibitors, Alzheimer disease, cholinergic system,...
|
|
|
Evaluation of the kinetics of acetylcholinesterase inhibitors in vitro
Janská, Kateřina ; Herink, Josef (advisor) ; Hrdina, Radomír (referee)
Kateřina Janská Evaluation of the kinetics of acetylcholinesterase inhibitors in vitro Diploma thesis Charles University in Prag, Faculty of Pharmacy in Hradec Králové Pharmacy Department of Biological and Medical Sciences Supervisor: Doc. MUDr. Josef Herink, DrSc. Consultant: PharmDr. Vendula Šepsová The aim of the thesis was to determine the type of an inhibition of newly synthesized AChEI and to find out if AChEI structure changes influence the type of an inhibition. Altogether 12 substances (7 tacrine hybrides and 5 7-methoxy- donepezil hybrids) were investigated. The inhibition potential of the tested substances was studied in vitro on the human recombinant AChE. Spectrophotometric Ellman method was utilized as the measurement tool. The noncompetitive type of an inhibition for substances EN 1-5, PC-25 and PC-33, mixed type of an inhibition for substances PC-48 and PC-49, uncompetitive type of an inhibition for substances EN-6, EN-7 and competitive type of an inhibition for the substance PC-37 was determined. The greatest inhibition potential according to Ki values were found for substances EN-7 and PC-37. Substances PC-37 and PC-48 were determined as substances with the biggest affinity to the AChE. The type of an inhibition has been influenced by a substituent position in PC substances and by...
|
|
|
Signs of the temporolimbic dysfunction in persons with headaches
Bartošová, Tereza ; Herink, Josef (advisor) ; Kudláčková, Zděnka (referee)
The aim of this research is the occurrence of symptoms of the limbic dysfunction in patients suffering from headaches. By means of the questionnaire survey method was tested group of people with this diagnosis. The basic premise is the assumption that certain symptoms, specifically abnormalities in brain activity, occur more frequently in patients with this diagnosis. The theoretical part of this thesis concentrates on the function of the temporal lobe and the limbic system, a brief outline of the characteristics of temporo-limbic dysfunction and a summary overview of headaches. The research part is addressed to the evaluation questionnaire study and answers to the questions asked. Keywords Temporal lobe, limbic system, temporolimbic dysfunction, headaches, migraine, comorbidity,questionnaire
|
|
|
Deep vein thrombosis of lower limbs - the therapy of thrombosis in women using alteplase
Prokšová, Magdaléna ; Kudláčková, Zděnka (advisor) ; Herink, Josef (referee)
Charles Unversity in Prague Faculty of pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Magdaléna Prokšová Supervisor: PhDr. Zdeňka Kudláčková Ph.D. Consultant: doc. MUDr. Radovan Malý Ph.D. Title of Thesis: Deep vein thrombosis of lower limbs - the therapy of thrombosis in women using alteplase Background: The aim of study was observed undesirable effects of treatment of deep vein thrombosis, than safety and how long lasting an intervetion, incidence of deep vein thrombosis on lower limbs, different ages and risk factors conecting with this disease too. Methods: In this study we administred Alteplase - a thrombolytic drug - directly to the vein using a catheter. Alteplase is a tissue plasminogen activator and it activates the transformation of plasminogen to plasmin and than plasmin breaks down blood clots and helps to renew the blood floow in the affected vessel. The thrombolytic drug was administered directly to the thrombus with using catether. In this study the 68 women were treated regardless of their age. Blood sample from pacients took away before, during and after inervention to set down levels of platelets and fibrinogen. Result: Deep vein thrombosis occured mostly in women of younger ages on their right lower limb. Bleeding manifestations were the most...
|
|
|
Screening of p16 protein level, a potencial marker for cervical cancer
Hedlová, Kateřina ; Konečná, Klára (advisor) ; Herink, Josef (referee)
Author: Kateřina Hedlová Title: Screening of p16 protein level, a potencial marker for cervical cancer Bachelor's thesis Charles University in Prague Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Degree program: Medical bioanalysis Study: Medical laboratory technician Background: Cervical cancer is one of the few oncological disease with proven etiological agent. The cause of this disease is persistent infection caused by high-risk genotypes of human papillomavirus . Cervical cancer is worldwide the second most common cause of death in women under 45 years of age. Preventive gynecological examinations, which include a cytologicalexamination of the cervix can soon diagnose pre-cancerous stages and thus significantly contribute to the timely treatment.. At present, commonly used screening tests are primarily limited in senzitivity, therefore are searched for more senzitive and specific new diagnostic methods.One of the candidate methods is test based on quantitative determination of p16 by ELISA method. Methods: Colorimetric enzyme linked immunoanalysis, a commercially available kit CervatecTM p16INK4a ELISA. Result: 72 various cytological smears from cervix were obtained from gynecological examinations of women. Determined values of protein p16INK4a were in 65...
|
|
|
Acute intoxication in urgent medicine
Průšová, Kristýna ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of pharmacology and toxicology Candidate: Mgr. Kristýna Průšová Consultant: PharmDr. Marie Vopršalová, CSc. Title of Thesis: Acute intoxication in urgent medicine This rigorous work is devided on the theoretical and experimental part. The theoretical part describes the effect of toxic substances on health. It also describes and sums up the latest ways of diagnostics and treatment of intoxication. At the end it concerned with the description of the individual types of intoxication covered in the experimental part. The aim of this work is to statistically evaluate data about intoxicated patients accepted by College Hospital Pilsen from January 2010 to April 2013. It is connected with the aim of finding link among the term of intoxication, the age and sex of intoxicated patients, the way of exposition, the type of intoxication, the reason of intoxication and the prognosis. Among aims there is also to find dependence between consumed medicaments and above stated factors. The data were firstly described by basic descriptive characteristics, then the differences among them were tested. Relations among dependent and independent factors were tested using multidimensional analyses. Results of analyses were presented in unimodal...
|
|
|
JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease
Kohoutová, Lucie ; Rathouská, Jana (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Lucie Kohoutová Supervisor: PharmDr. Jana Rathouská, Ph. D. Specialized supervisor: Dr. Maria Javier Ramirez Title of diploma thesis: JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease Background: The pathogenesis of Alzheimer's disease (AD) is incompletely understood. Discovery of c-Jun N-terminal kinases (JNKs), involved in regulation of gene expression, inflammation, cell proliferation and apoptosis, may contribute to better understanding of AD pathogenesis. JNK3 is a brain-specific JNK isoform involved in apoptosis of mammalian neurons. Precise localization of JNK3 in the brain still remains unclear. JNK3 silencing achieved by gene knockout had directly decreased beta amyloid (Aβ) levels in previous AD models in mice. We performed an immunohistological study to localize JNK3 gene in the mice brain in order to better focus future gene targeting therapy. Methods: A transgenic mice model of AD (Tg2576) has been used to study the expression of JNK3 by western blotting compared to the wild type. In the same brains localization of pJNK3 in astroglia (GFAP), microglia (Ox42), neurons (NeuN), senile plaques (Aβ) or pTau, using immunohistochemistry staining...
|
guest ::
RSS feed.