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Epigenetic regulation of expression of immunoactive genes on tumor cells
Hejhal, Tomáš ; Reiniš, Milan (advisor) ; Šmahel, Michal (referee)
The aim of this master thesis was to identify epigenetically silenced genes and to determine molecular effects of DNA methyltransferases inhibitor (5-aza-2'-deoxycytidine) and histon deacetylases inhibitor trichostatin A (TSA) on gene expression in cancer cell lines TC-1/A9 and RVP3. Epigenetic events play an important role in tumorigenesis and also in escape of cancer cells from immune surveillance. I analyzed global changes in gene expression profiles of two cell lines by microarray analysis with a special attention paid to immunoactive genes. The experimental model used for this purpose were murine tumor cell lines, MHC class I deficient. I identified epigenetic regulation of several genes that are involved in cancer and immune system interactions. These data demonstrate that epigenetic agents are capable to activate expression of genes that are important for antigen presentation, cell adhesion and apoptosis. Powered by TCPDF (www.tcpdf.org)
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Epigenetic regulation of expression of immunoactive genes on tumor cells
Hejhal, Tomáš ; Reiniš, Milan (advisor) ; Šmahel, Michal (referee)
The aim of this master thesis was to identify epigenetically silenced genes and to determine molecular effects of DNA methyltransferases inhibitor (5-aza-2'-deoxycytidine) and histon deacetylases inhibitor trichostatin A (TSA) on gene expression in cancer cell lines TC-1/A9 and RVP3. Epigenetic events play an important role in tumorigenesis and also in escape of cancer cells from immune surveillance. I analyzed global changes in gene expression profiles of two cell lines by microarray analysis with a special attention paid to immunoactive genes. The experimental model used for this purpose were murine tumor cell lines, MHC class I deficient. I identified epigenetic regulation of several genes that are involved in cancer and immune system interactions. These data demonstrate that epigenetic agents are capable to activate expression of genes that are important for antigen presentation, cell adhesion and apoptosis. Powered by TCPDF (www.tcpdf.org)
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Analysis of the state of information security in the environment of municipality Strakonice
Hejhal, Tomáš ; Doucek, Petr (advisor) ; Veber, Jaromír (referee)
This thesis is about information system analysis of municipality Strakonice with focus on its security side in comparison with security standards ISO/IEC 27000. This thesis has three main parts. The first part is about theory to the topic, respective about organization's basic information and relevant laws and standards. Second part include information system risk analysis of city Strakonice with suggestions reducing individual risks with establishing countermeasures or this risk providing with insurance. In case of low risk can be this risks accepted. Third part is about current state of information security. Benefits and purpose of this thesis is to write up risk analysis for IS of municipality Strakonice and write up analysis of current state of information security with recommendations for improvement.
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