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The activity of biotransformation enzymes in mouflons with dicrocoeliosis and in healthy mouflons
Urbánková, Marie ; Szotáková, Barbora (advisor) ; Gavelová, Martina (referee)
Charles University Faculty of Pharmacy Department of Biochemical Sciences Hradec Králové, Czech Republic Field: Patobiochemistry and xenobiochemistry Candidate: Mgr. Marie Urbánková Consultant: Ing. Barbora Szotáková, Ph.D. Title: The activity of biotransformation enzymes in mouflons with dicrocoeliosis and in healthy mouflons Abstract In vitro activities of cytochromes P450 (7-alkyl/aryloxyresorufin dealkyl(aryl)ases, testosterone hydroxylase/oxidase, 7-methoxy-4-trifluoromethyl-coumarin demethylase), flavine monooxygenases (toward thiobenzamide), reductases of carbonyl group (toward oracin, 4-pyridine-carboxaldehyde, 1-acenaphthenol, DL-glyceraldehyde, ketoprofene, naloxone, daunorubicin, metyrapone) and cojnugation enzymes (p-nitrophenol-UDP- glukuronosyl transferase, UGT, 1-chloro-2,4-dinitrobenzene glutathione-S-transferase, GST) in old, female mouflons (Ovis musimon, Bovidae), both in healthy ones and those suffered from dicrocoeliosis were studied and compared. This parasitary disease is caused by Dicrocoelium dendriticum. Various methods were used, especially spectrophotometry, spectrofluorimetry, and method based on incubation of enzymes with relative specific substrate followed with HPLC analysis of biotransformation products. The amount of proteins was determined using methods with reduction of...
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Human paraoxonase (PON1) L55M allelic polymorphism and hemodialysis
Dvořáková, Renata ; Dršata, Jaroslav (advisor) ; Gavelová, Martina (referee)
Allelický polymorfismus lidské paroxonasy a hemodialýza Renata Dvořáková ABSTRAKT Nemocní léčení hemodialýzou (HD) mají vysoké riziko koronárního onemocnění. Lidská paraoxonasa (PON1, EC 3.1.8.1) je enzym asociovaný s lipoproteinem o vysoké hustotě (HDL), který chrání lipoprotein LDL před oxidací a rovněž chrání před aterosklerózou. Na vzniku CHD se může účastnit polymorfismus PON1 L55M. Cíl práce: Zmapovat distribuci allelického polymorfismu PON1 L55M v Chorvatsku a určit vztah mezi polymorfismem PON1 L55M a hemodialytickou léčbou . Metody: Do studie byli zahrnuti jedinci HD-pacienti (N=51, M/F= 28/23, věk= 60±12 let) a kontrolní jedinci (dárci krve) (N=95, M/F=48/47, věk 49±17 let) z oblasti Slavonski Brod. Polymorfismus PON1 L55M byl zjišťován metodami PCR a metodou sledování délky fragmentů polymorfismu (RFLP). Výsledky: Distribuce polymorfismu genotypů PON1 L55M u pacientů s hemolýzou se lišila od kontrol (12%MM, 48% LM a 40% LL). Frekvence polymorfismu allel pro PON1 L55M se lišila v 5% u hemodialyzovaných (69% L allela, 31% M allela) a kontrol (64% L, 36% M). L allela je nejčastější jak u skupiny HD, tak u kontrol. Závěr: Přibližně 55,5 polymorfických jedinců s PON1 L55M a 44,5 jedinců bez polymorfismu PON1 L55M. L allela je nejčastější u polymorfismu PON1 L55M u obou porovnávaných skupin. Frekvence...
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Developmental expression pattern of S1co1c1
Hroudová, Jana ; Wsól, Vladimír (advisor) ; Gavelová, Martina (referee)
in English language Introdution Thyroid hormones are necessary for the development of many organs and systems in the body, including the central nervous system. The association between thyroid function and development of the brain is well known, depressed function of thyroid gland and its lower production can lead to various neurological deficits and disorders. During the development of the fetus it can result to mental retardation, ataxia and blindness. Methods Slco1c1-Cre mice were anesthetized and perfused. After their decapitation brains were carefully dissected and fixed in 4% PFA at 4řC for 4-12 hours and then transferred into 30% Sucrose solution for 24 hours. Sections were prepared with cryotome, collected on the slides covered with polysine and into two series. They were parallelly stained with lacZ staining and Nissl staining. LacZ showed the expression of Slco1c1-Cre, Nissl staining was performed as additive staining and distinguished different structures in the brain. The expressions were observed unter the microscope and were evaluated in tables. The presence of Slco1c1 was observed similarly in P2 generation. Results Both staining methods were standardized, incubation time was settled for lacZ staining and the performance of Nissl staining was improved. The strongest expression of lacZ...
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Human paraoxonase (PON1) L55M allelic polymorphism and hemodialysis
Dvořáková, Renata ; Dršata, Jaroslav (advisor) ; Gavelová, Martina (referee)
Allelický polymorfismus lidské paroxonasy a hemodialýza Renata Dvořáková ABSTRAKT Nemocní léčení hemodialýzou (HD) mají vysoké riziko koronárního onemocnění. Lidská paraoxonasa (PON1, EC 3.1.8.1) je enzym asociovaný s lipoproteinem o vysoké hustotě (HDL), který chrání lipoprotein LDL před oxidací a rovněž chrání před aterosklerózou. Na vzniku CHD se může účastnit polymorfismus PON1 L55M. Cíl práce: Zmapovat distribuci allelického polymorfismu PON1 L55M v Chorvatsku a určit vztah mezi polymorfismem PON1 L55M a hemodialytickou léčbou . Metody: Do studie byli zahrnuti jedinci HD-pacienti (N=51, M/F= 28/23, věk= 60±12 let) a kontrolní jedinci (dárci krve) (N=95, M/F=48/47, věk 49±17 let) z oblasti Slavonski Brod. Polymorfismus PON1 L55M byl zjišťován metodami PCR a metodou sledování délky fragmentů polymorfismu (RFLP). Výsledky: Distribuce polymorfismu genotypů PON1 L55M u pacientů s hemolýzou se lišila od kontrol (12%MM, 48% LM a 40% LL). Frekvence polymorfismu allel pro PON1 L55M se lišila v 5% u hemodialyzovaných (69% L allela, 31% M allela) a kontrol (64% L, 36% M). L allela je nejčastější jak u skupiny HD, tak u kontrol. Závěr: Přibližně 55,5 polymorfických jedinců s PON1 L55M a 44,5 jedinců bez polymorfismu PON1 L55M. L allela je nejčastější u polymorfismu PON1 L55M u obou porovnávaných skupin. Frekvence...
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The activity of biotransformation enzymes in mouflons with dicrocoeliosis and in healthy mouflons
Urbánková, Marie ; Szotáková, Barbora (advisor) ; Gavelová, Martina (referee)
Charles University Faculty of Pharmacy Department of Biochemical Sciences Hradec Králové, Czech Republic Field: Patobiochemistry and xenobiochemistry Candidate: Mgr. Marie Urbánková Consultant: Ing. Barbora Szotáková, Ph.D. Title: The activity of biotransformation enzymes in mouflons with dicrocoeliosis and in healthy mouflons Abstract In vitro activities of cytochromes P450 (7-alkyl/aryloxyresorufin dealkyl(aryl)ases, testosterone hydroxylase/oxidase, 7-methoxy-4-trifluoromethyl-coumarin demethylase), flavine monooxygenases (toward thiobenzamide), reductases of carbonyl group (toward oracin, 4-pyridine-carboxaldehyde, 1-acenaphthenol, DL-glyceraldehyde, ketoprofene, naloxone, daunorubicin, metyrapone) and cojnugation enzymes (p-nitrophenol-UDP- glukuronosyl transferase, UGT, 1-chloro-2,4-dinitrobenzene glutathione-S-transferase, GST) in old, female mouflons (Ovis musimon, Bovidae), both in healthy ones and those suffered from dicrocoeliosis were studied and compared. This parasitary disease is caused by Dicrocoelium dendriticum. Various methods were used, especially spectrophotometry, spectrofluorimetry, and method based on incubation of enzymes with relative specific substrate followed with HPLC analysis of biotransformation products. The amount of proteins was determined using methods with reduction of...
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