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Analysis of the retinal nerve fiber layer in hiv positive patients in era of combination antiretroviral treatment
Kožner, Pavel ; Filouš, Aleš (advisor) ; Kalvodová, Bohdana (referee) ; Sedláček, Dalibor (referee)
The aim of the study was to evaluate the effect of human immunodeficiency virus infection (HIV) and antiretroviral treatment on the retinal nerve fibre layer (RNFL). The RNFL hickness defined by standard parameters(TSNIT average, Superior average and Inferior average) was assessed in 48 HIV positive patients using scanning laser polarimeter, GDx VCC device. Results were compared to normal values and tested against factors suspected to affect the RNFL thickness. The mean values of the RNFL standard parameters were for TSNIT average, Superior average and Inferior average, 57,65 ± 6,18 m, 69,38 ± 8,34 m, 68,89 ± 9,50 m respectively, in our cohort. The RNFL thinning was not confirmed in our HIV positive group compared to values on healthy population. No significant correlation between the RNFL thickness and the immune profile or antiretroviral therapy was detected. However, a significant negative correlation between the RNFL thickness with increasing duration of HIV infection was foundin our study that is hypothesized to be possibly on an immune pathological basis. Powered by TCPDF (www.tcpdf.org)
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Retinal nerve fiber layer thickness in pediatric glaucoma patients, measured by scanning laser polarimetry
Hložánek, Martin ; Filouš, Aleš (advisor) ; Autrata, Rudolf (referee) ; Bělohlávek, Otakar (referee)
Martin Hložánek: Retinal nerve fiber layer thickness in pediatric glaucoma patients, measured by scanning laser polarimetry Abstract Introduction Glaucoma is the leading cause of irreversible blindness worldwide. It is characterized as progressive neuropathy of retinal ganglion cells. Diagnosis of glaucoma can be difficult and often requires several different investigations, performed repeatedly. Evaluation of thickness of retinal nerve fiber layer (RNFL) by scanning laser polarimetry (with GDxVCC machine) belongs to the most sensitive objective methods of glaucoma diagnostics and monitoring and it is also easy to perform. Importance of this method is even higher in children, as the performance of other diagnostic methods is difficult due to poor cooperation. Normative databases of machines providing objective measurement of RNFL thickness, including GDxVCC, don't comprise data of subjects younger 18 years. Methods The aims of our work were to assess normative values of RNFL parameters on GDxVCC in healthy children and to compare them with values of children diagnosed with glaucoma. We performed measurements of RNFL thickness and basic ophthalmological testings in 186 children of age 7-17 years. During our study there were published papers pointing the influence of atypical birefringence pattern (ABP) on...
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Analysis of the retinal nerve fiber layer in hiv positive patients in era of combination antiretroviral treatment
Kožner, Pavel ; Filouš, Aleš (advisor) ; Kalvodová, Bohdana (referee) ; Sedláček, Dalibor (referee)
The aim of the study was to evaluate the effect of human immunodeficiency virus infection (HIV) and antiretroviral treatment on the retinal nerve fibre layer (RNFL). The RNFL hickness defined by standard parameters(TSNIT average, Superior average and Inferior average) was assessed in 48 HIV positive patients using scanning laser polarimeter, GDx VCC device. Results were compared to normal values and tested against factors suspected to affect the RNFL thickness. The mean values of the RNFL standard parameters were for TSNIT average, Superior average and Inferior average, 57,65 ± 6,18 m, 69,38 ± 8,34 m, 68,89 ± 9,50 m respectively, in our cohort. The RNFL thinning was not confirmed in our HIV positive group compared to values on healthy population. No significant correlation between the RNFL thickness and the immune profile or antiretroviral therapy was detected. However, a significant negative correlation between the RNFL thickness with increasing duration of HIV infection was foundin our study that is hypothesized to be possibly on an immune pathological basis. Powered by TCPDF (www.tcpdf.org)
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Radical injury of cornea and lens
Mahelková, Gabriela ; Novotná, Jana (advisor) ; Filouš, Aleš (referee) ; Čejková, Jitka (referee)
We studied whether hypoxia of corneal tissue increases the collagenolytic activity due to release of reactive oxygen and nitrogen species. We found no differences in the corneal tissue in the gel electrophoretic profile of collagenous proteins and gelatinolytic activity between normoxic and hypoxic rats. We did not find any sign of radical tissue injury. There were no changes in the vascularization of corneas after exposition to hypoxia. The environmental 10 % hypoxia does not induce radical tissue injury and an increase of collagenolytic activity in the rat cornea. We studied the effect of several culture substrates and culture conditions on lens epithelial cells (LEC) proliferation and -smooth muscle actin (-SMA) expression. There was no difference in growth characteristics of primary cultures on different collagen substrates and plastic dishes. The cells growth worse on the glass. In secondary cultures, LECs adhered better to collagen-coated surfaces. The culture substrate influenced LEC proliferation and -SMA expression. The proliferation was greater when the medium was changed than when extra medium was added on the 4th day. The cells did not synthesize -, - or -crystallin. It is necessary to consider the effects of the medium exchange protocol, serum supplementation, cell density and other cell...
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