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National Repository of Grey Literature

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	Produkce polyklonálních protilátek proti rekombinantnímu proteinu X viru bramboru Čeřovská, Noemi ; Plchová, Helena ; Moravec, Tomáš ; Filigarová, Marie ; Dědič, P. The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX in laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot. Detailed record
	Genetic Variability Between Different Isolates of Potato Mop-top Virus (PMTV) Rosecká, Pavla ; Moravec, Tomáš ; Filigarová, Marie ; Dědič, P. ; Čeřovská, Noemi We determined partial nucleotide sequences of several different field isolates of PMTV with well-characterized biological properties from the Czech Republic and Denmark. Subsequently we were trying to elucidate the correlation between nucleotide sequences of various isolates, their biological properties and geographical origin. From the comparison with other known sequences we can conclude, that PMTV genome is relatively conserved. Detailed record
	Serological and biological differentiation of isolates of the potato virus S Filigarová, Marie ; Čeřovská, Noemi ; Dědič, P. Detailed record
	Monoclonal antibodies to potato virus S (Andean strain) Čeřovská, Noemi ; Filigarová, Marie Detailed record

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