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Voltammetric Determination of DNA Damage Caused by Genotoxic 2-Aminofluorene at a Carbon Film Electrode
Faťarová, Mária ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
A large-surface carbon film electrode (ls-CFE) represents a suitable alternative to the commercially available disposable screen-printed carbon working electrodes. Its simple, fast and inexpensive preparation, simple mechanical renewal of the electrode surface (by wiping off the old film with filter paper and forming a new one), good repro- ducibility of measurements, elimination of problems connected with "electrode history" and simple chemical modification are its main advantages. In this Bachelor Thesis, the utilization of ls-CFE for the preparation of a simple elec- trochemical DNA biosensor for the detection of carcinogenic environmental pollutant, 2-aminofluorene (2-AF), is presented. The composition of carbon ink suspension was optimized and tested using a Fe(II)/Fe(III) redox system by cyclic voltammetry (CV). A large-surface solid electrode, covered by a carbon film of the optimum composition, was then used as a transducer for the preparation of double stranded DNA (dsDNA) based electrochemical DNA biosensor (dsDNA/ls-CFE). The electrochemical behavior of dsDNA (i) present in the measured supporting electrolyte (0.1 mol.L−1 acetate buffer of pH 4.7) or (ii) attached at the ls-CFE surface (by covering with dsDNA stock solution and leaving to dry) was investigated using square wave voltammetry...
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Analýza sexuálního feromonu ovocných mušek rodu Ceratitis fasciventris, Ceratitis anonae a Ceratitis rosa
Faťarová, Mária ; Kalinová, Blanka (advisor) ; Rezek, Jan (referee)
Ceratitis fasciventris, Ceratitis anonae and Ceratitis rosa are polyphagous agricultural pests originating from African continent. Their behaviour is heavily altered by pheromones. Insect chemical communication channels are species-specific, represents taxonomic and reproduction barriers. Taxonomy of this group (so-called Ceratitis FAR complex) is unclear. Therefore new chemical approaches along with genetic tests for identification of entities within the cryptic species FAR complex are being developed. To study multi-component mixtures of male volatiles originating from the three mentioned fruit fly species, comprehensive two-dimensional gas chromatography with time- of-flight mass spectrometer (GC×GC-TOFMS) was used. A number of compounds were identified, out of which 23 were found to be distributed and shared among the studied species, 11 out of these were present in all three species. Analyses of male pheromone volatiles, using gas chromatography combined with electroantennographic detector (GC-EAD) revealed 4 common compounds with antennal activity shared among three studied species: methyl (E)-hex-3-enoate, 6-methylhept-5-en-2- one, linalool, and methyl (2E,6E)-farnesoate. The species-specific EAD active compounds in C. fasciventris were esters of isomers of hexenoic acid, whereas isomers of...
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Voltammetric Determination of DNA Damage Caused by Genotoxic 2-Aminofluorene at a Carbon Film Electrode
Faťarová, Mária ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
A large-surface carbon film electrode (ls-CFE) represents a suitable alternative to the commercially available disposable screen-printed carbon working electrodes. Its simple, fast and inexpensive preparation, simple mechanical renewal of the electrode surface (by wiping off the old film with filter paper and forming a new one), good repro- ducibility of measurements, elimination of problems connected with "electrode history" and simple chemical modification are its main advantages. In this Bachelor Thesis, the utilization of ls-CFE for the preparation of a simple elec- trochemical DNA biosensor for the detection of carcinogenic environmental pollutant, 2-aminofluorene (2-AF), is presented. The composition of carbon ink suspension was optimized and tested using a Fe(II)/Fe(III) redox system by cyclic voltammetry (CV). A large-surface solid electrode, covered by a carbon film of the optimum composition, was then used as a transducer for the preparation of double stranded DNA (dsDNA) based electrochemical DNA biosensor (dsDNA/ls-CFE). The electrochemical behavior of dsDNA (i) present in the measured supporting electrolyte (0.1 mol.L−1 acetate buffer of pH 4.7) or (ii) attached at the ls-CFE surface (by covering with dsDNA stock solution and leaving to dry) was investigated using square wave voltammetry...
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