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Determination of platelet activation markers expression in the pathophysiology of thrombotic states
Řádek, Martin ; Kvasnička, Tomáš (advisor) ; Dulíček, Petr (referee) ; Říhová, Lucie (referee)
Background/Aims: Thrombophilia, ie an increased predisposition to venous and arterial thrombosis, is a complex disease caused by disorders of platelets and endothelial, among others. Circulating endothelial cells (CEC) and endothelial progenitor cells (EPC) have been described as markers of endothelial damage and dysfunction, respectively as markers of its recovery in many diseases, including thrombotic complications. However, their significance in patients with known thrombophilia has not yet been investigated. Both CEC and EPC represent extremely rare cell populations found in peripheral blood. Therefore, it is essential to use exclusively standardized and sensitive methods for their identification and quantification. The aim of the study was to identify and quantify CEC and EPC in the peripheral blood of patients with congenital thrombophilia and to evaluate their importance as markers of endothelial and platelet activity in context with the risk of thrombosis occuring and recurrence. Methods: Analysis of the number of CEC and EPC in the peripheral blood of patients with thrombophilia with or without a history of thrombosis and patients with acute thrombosis was performed by multicolor flow cytometry. The CEC and EPC reference values were determined on a group of healthy controls. Patients with...
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Cerebral venous thrombosis risk factors and JAK2 mutation
Košťál, Milan ; Dulíček, Petr (advisor) ; Doubek, Michael (referee) ; Krčová, Věra (referee)
Cerebral venous thrombosis risk factors and JAK2 mutation Cerebral venous thrombosis is a rare type of cerebrovascular disease. The widespread use of neuroimaging has aided early diagnosis of CVT and its parenchymal complications. Etiology and natural history of CVT differs from DVT mainly due to different pathophysiologic conditions of sinuses. The main objective of this study was to find out prevalence of genetic and acquired thrombophilic factors and prevalence of JAK2 V617F mutation (typical for myeloproliferative diseases) in CVT patients and discover their relation to prognosis and treatment. Next target of our survey was to identify typical symptoms of CVT and to verify significance of clinical indicators (time to diagnosis, type of therapy, recurrence, recanalization, etc.) and laboratory tests (D-dimers, blood count). Minority of the data were collected as retrospective (5 patients from a hospital in Pardubice, 18 patients from the University hospital in Hradec Králové). Majority of study was prospective; all patients with CVT prior to the starting point of survey were re-evaluated in outpatient department during the study. Healthy controls were recruited from Transfusion department of the University hospital in Hradec Králové We enrolled 64 patients and 70 healthy controls. JAK2 mutation was more...
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