National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Use of microbial community structure as a marker of conditions in underground biotops
Burkartová, Kateřina ; Falteisek, Lukáš (advisor) ; Drahota, Petr (referee)
The amount of data obtained by barcoding of prokaryotic 16S rDNA from natural habitats is increasing exponentially. Thus, methods enabling us to extract useful information from these data are of increasing importance. In this thesis microbial communities from water, sludge and drilling dust were analyzed by 16S rDNA sequencing in three geologically well described sedimentary aquifers in Bohemian Massif. The main goal of this research was to establish how different analytical approaches can be useful in interpretation of groundwater biogeochemical processes. Three approaches were used: First, taxonomy and metabolic traits of the most abundant microorganisms were assessed. Second, ordination methods showing metabolic and taxonomic variability between communities were used. Last the analysis of phylogenetic dissimilarity using UniFrac metrics was performed. When analyzing individual localities separately, the shift in microbial community composition corresponds with the change of environmental conditions. The unconstrained ordination method based on the variability in metabolic traits indicated, that sludge samples are more informative than water samples when asking which electron donor is used in microbial communities. On the other hand, unconstrained ordination methods were useless when the...
Microorganisms as the primary producers in photosynthesis-independent ecosystems
Burkartová, Kateřina ; Falteisek, Lukáš (advisor) ; Lhotský, Josef (referee)
Chemolithotrophy and photoautotrophy are the two basic principles of primary production on Earth. This thesis focuses on the relation between chemolithotrophic and oxygen-productive photosynthetic microorganisms in a global dimension. There are high atmospheric oxygen concentration, high sulfate in seawater and other oxidants because of oxygen-productive photosynthesis. These compounds are commonly consumed by chemolithotrophs to oxidize reduced inorganic compounds. It is a question, if there are chemoautotrophic ecosystems profiting only from abiotically derived substrates. It is essential to combine geochemical and metagenomic approaches in order to enhance both establishing of the origin of substrates and assessing the metabolism which utilizes them in the tested ecosystem respectively. Two well established types of photosynthetic independent ecosystems that use serpentinization hydrothermal venting and radiolysis of water as a source of energy are known at present time. The absence of photosynthesis-derived final electron acceptors for chemolithotrophy and the proper spatial separation from areas where photosynthetically derived organic matter and oxidants are present can be a useful clue for this investigation. The goal of this thesis is to describe geological processes which provide not only...

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